@article{48e14af9490542eba09591b6d29415f2,
title = "Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation",
abstract = "Inflammation mediates tissue repair but can be hijacked to promote tumorigenesis. Hoste et al. demonstrate that HMGB1 delays regeneration and drives tumor formation in skin by recruitment and priming of neutrophils. These data indicate that therapies targeting HMGB1 or NET formation might be relevant in chronic and diabetic wound treatment.",
keywords = "HMGB1, TNF, diabetes, epidermolysis bullosa, innate immunity, neutrophil extracellular traps, skin inflammation, tumor microenvironment, wound healing",
author = "Esther Hoste and Christian Mauer{\"o}der and {van Hove}, Lisette and Leen Catrysse and Vikkula, {Hanna Kaisa} and Mozes Sze and Bastiaan Maes and Dyah Karjosukarso and Liesbet Martens and Amanda Gon{\c c}alves and Eef Parthoens and Ria Roelandt and Wim Declercq and Ignacia Fuentes and Francis Palisson and Sergio Gonzalez and Salas-Alanis, {Julio C.} and Louis Boon and Peter Huebener and Mulder, {Klaas Willem} and Kodi Ravichandran and Yvan Saeys and Schwabe, {Robert Felix} and {van Loo}, Geert",
note = "Funding Information: We thank Ruben Van Renterghem and Sabine Cleemput for animal care and acknowledge the VIB BioImaging Core, the VIB Nucleomics Core, the VIB FlowCore, and Gert Van Isterdael for technical assistance. TLR-4 blocking antibody was kindly provided by Dr. Walter Ferlin. We thank Manolis Pasparakis for providing RIPK1D138N mice and all members of the van Loo laboratory for suggestions and discussions. E.H. is supported by an FWO postdoctoral fellowship. L.v.H. holds an FWO FR fellowship. Research in the van Loo lab is supported by research grants from the FWO, the Concerted Research Actions (GOA) of Ghent University, the Geneeskundige Stichting Koningin Elisabeth (GSKE), Stichting tegen Kanker, the CBC Banque prize, the Charcot Foundation, the Belgian Foundation against Cancer, and Kom op tegen Kanker. C.M. is supported by a postdoctoral research fellowship from the German Research Foundation (DFG) (MA 7770/1-1). We are grateful for the courtesy of Peter Vandenabeele to deliver purified HMGB1 in the framework of a Methusalem grant (BOF16/MET_V/007). W.D. lab is supported by Stichting tegen Kanker (FAF-F/2016/868). I.F. is funded by FONDECYT (1181093). K.R. is supported by an Odysseus grant and ERC Advanced Grant. E.H. conceptualized the study, designed and performed experiments, and analyzed the data. C.M. L.v.H. L.C. and H.-K.V. performed experiments. I.F. F.P. S.G. and J.C.S.-A. provided skin tissue sections from normal and RDEB patients. B.M. A.G. and E.P. performed and analyzed live imaging experiments. R.R. and W.D. provided ultrapure Box A protein. D.K. and K.W.M. provided supernatants from human cell lines. L.B. provided anti-TNF antibody. P.H. and R.F.S. provided the “floxed” HMGB1 mice. L.C. L.M. Y.S. K.R. and G.v.L. analyzed the data and provided input for experimental design. E.H. C.M. and G.v.L. wrote the manuscript with input from all authors. The authors declare no competing interests. Funding Information: We thank Ruben Van Renterghem and Sabine Cleemput for animal care and acknowledge the VIB BioImaging Core, the VIB Nucleomics Core, the VIB FlowCore, and Gert Van Isterdael for technical assistance. TLR-4 blocking antibody was kindly provided by Dr. Walter Ferlin. We thank Manolis Pasparakis for providing RIPK1 D138N mice and all members of the van Loo laboratory for suggestions and discussions. E.H. is supported by an FWO postdoctoral fellowship. L.v.H. holds an FWO FR fellowship. Research in the van Loo lab is supported by research grants from the FWO , the Concerted Research Actions (GOA) of Ghent University , the Geneeskundige Stichting Koningin Elisabeth (GSKE), Stichting tegen Kanker , the CBC Banque prize, the Charcot Foundation , the Belgian Foundation against Cancer , and Kom op tegen Kanker . C.M. is supported by a postdoctoral research fellowship from the German Research Foundation (DFG) ( MA 7770/1-1 ). We are grateful for the courtesy of Peter Vandenabeele to deliver purified HMGB1 in the framework of a Methusalem grant ( BOF16/MET_V/007 ). W.D. lab is supported by Stichting tegen Kanker ( FAF-F/2016/868 ). I.F. is funded by FONDECYT ( 1181093 ). K.R. is supported by an Odysseus grant and ERC Advanced Grant. Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = nov,
day = "26",
doi = "10.1016/j.celrep.2019.10.104",
language = "English",
volume = "29",
pages = "2689--2701.e4",
journal = "Cell Reports",
issn = "2211-1247",
number = "9",
}