Epithelial attachment alters the outcome of Helicobacter pylori infection

Janaki L. Guruge; Per G. Falk; Robin G. Lorenz; Maria Dans; Hans Peter Wirth; Martin J. Blaser; Douglas E. Berg; Jeffrey I. Gordon

doi:10.1073/pnas.95.7.3925

Epithelial attachment alters the outcome of Helicobacter pylori infection

Janaki L. Guruge, Per G. Falk, Robin G. Lorenz, Maria Dans, Hans Peter Wirth, Martin J. Blaser, Douglas E. Berg, Jeffrey I. Gordon

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254 Scopus citations

Abstract

Genetically defined in vivo models are needed to assess the importance of target cell attachment in bacterial pathogenesis. Gastric colonization by Helicobacter pylori in human populations is common and persistent, and has various outcomes including peptic ulcers and cancer. The impact of attachment on the course of infection was examined in transgenic mice expressing a human receptor for H. pylori in their gastric epithelium. Persistent infection by a clinical isolate occurred at comparable microbial densities in transgenic and nontransgenic littermates. However, microbial attachment in transgenic mice resulted in production of autoantibodies to Lewis(x) carbohydrate epitopes shared by bacteria and acid-secreting parietal cells, chronic gastritis, and parietal cell loss. This model should help identify bacterial and host genes that produce attachment-related pathology.

Original language	English
Pages (from-to)	3925-3930
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	95
Issue number	7
DOIs	https://doi.org/10.1073/pnas.95.7.3925
State	Published - Mar 31 1998

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title = "Epithelial attachment alters the outcome of Helicobacter pylori infection",

abstract = "Genetically defined in vivo models are needed to assess the importance of target cell attachment in bacterial pathogenesis. Gastric colonization by Helicobacter pylori in human populations is common and persistent, and has various outcomes including peptic ulcers and cancer. The impact of attachment on the course of infection was examined in transgenic mice expressing a human receptor for H. pylori in their gastric epithelium. Persistent infection by a clinical isolate occurred at comparable microbial densities in transgenic and nontransgenic littermates. However, microbial attachment in transgenic mice resulted in production of autoantibodies to Lewis(x) carbohydrate epitopes shared by bacteria and acid-secreting parietal cells, chronic gastritis, and parietal cell loss. This model should help identify bacterial and host genes that produce attachment-related pathology.",

author = "Guruge, {Janaki L.} and Falk, {Per G.} and Lorenz, {Robin G.} and Maria Dans and Wirth, {Hans Peter} and Blaser, {Martin J.} and Berg, {Douglas E.} and Gordon, {Jeffrey I.}",

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AU - Guruge, Janaki L.

AU - Falk, Per G.

AU - Lorenz, Robin G.

AU - Dans, Maria

AU - Wirth, Hans Peter

AU - Blaser, Martin J.

AU - Berg, Douglas E.

AU - Gordon, Jeffrey I.

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AB - Genetically defined in vivo models are needed to assess the importance of target cell attachment in bacterial pathogenesis. Gastric colonization by Helicobacter pylori in human populations is common and persistent, and has various outcomes including peptic ulcers and cancer. The impact of attachment on the course of infection was examined in transgenic mice expressing a human receptor for H. pylori in their gastric epithelium. Persistent infection by a clinical isolate occurred at comparable microbial densities in transgenic and nontransgenic littermates. However, microbial attachment in transgenic mice resulted in production of autoantibodies to Lewis(x) carbohydrate epitopes shared by bacteria and acid-secreting parietal cells, chronic gastritis, and parietal cell loss. This model should help identify bacterial and host genes that produce attachment-related pathology.

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Epithelial attachment alters the outcome of Helicobacter pylori infection

Abstract

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