Epidermal growth factor upregulates the expression of its own intestinal receptor after small bowel resection

Michael A. Helmrath, Cathy E. Shin, Christopher R. Erwin, Brad W. Warner

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Background/Purpose: Epidermal growth factor (EGF) binds to its enterocyte receptor and enhances intestinal adaptation after massive small bowel resection (SBR). To ascertain the mechanism for enhanced adaptation by EGF, we sought to determine the effect of EGF administration on in vivo expression of the intestinal EGF receptor after SBR. Methods: Male ICR mice underwent a 50% proximal SBR and then were assigned randomly to EGF (150 μg/kg/d) or saline by twice daily intraperitoneal injection. After 3 days, the ileum was harvested and total protein and DNA content were measured. Northern hybridization and a ribonuclease protection assay were used to detect qualitative and quantitative expression of EGF receptor mRNA. The remaining ileum was pooled for each group and Western blotting used to determine expression of EGF receptor protein. Results: EGF augmented adaptation after SBR as monitored by significant increases in ileal protein (2.7 ± 0.08 saline versus 3.9 ± 0.17 mg/cm EGF; P< .001) and DNA (55.8 ± 1.6 saline versus 104 ± 8.4 μgm/cm EGF; P< .001) content. Northern blotting results showed a marked (> fivefold) increase in ileal EGF receptor mRNA, which was confirmed with the ribonuclease protection assay. Administration of EGF after SBR induced a similar expression of EGF receptor protein. Conclusions: EGF enhanced intestinal adaptation after SBR. This augmented response is associated with increased ileal expression of EGF receptor mRNA and protein. Increased EGF receptor expression and subsequent enhanced ligand/receptor activity may be one important mechanism for the beneficial effect of EGF administration during intestinal adaptation.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalJournal of Pediatric Surgery
Volume33
Issue number2
DOIs
StatePublished - Feb 1998
Externally publishedYes

Keywords

  • Adaptation
  • EGF
  • Enterectomy
  • Mice
  • Receptor
  • Short bowel syndrome
  • Small bowel resection

Fingerprint Dive into the research topics of 'Epidermal growth factor upregulates the expression of its own intestinal receptor after small bowel resection'. Together they form a unique fingerprint.

  • Cite this