TY - JOUR
T1 - Epidermal growth factor alters the bax:bcl-w ratio following massive small bowel resection
AU - Stern, Lawrence E.
AU - Falcone, Richard A.
AU - Huang, Frederick
AU - Kemp, Christopher J.
AU - Erwin, Christopher R.
AU - Warner, Brad W.
N1 - Funding Information:
1 Supported by National Institutes of Health F32 DK09882 (LES), National Institutes of Health RO-1 DK53234 (BWW), and a grant from the Children’s Hospital Campaign for Children Fund, Children’s Hospital Medical Center, Cincinnati, OH.
PY - 2000/6/1
Y1 - 2000/6/1
N2 - Background. Following massive small bowel resection (SBR), the expression of bax and bcl-w is associated with increased enterocyte apoptosis. Epidermal growth factor (EGF) has been shown to enhance enterocyte proliferation and retard apoptosis in the adapting bowel. This study examined the effect of EGF on the expression of these bcl-2 family members during adaptation. Materials and methods. Mice (C57Bl/6; n = 38) underwent a 50% SBR or sham operation and then were randomized to receive twice-daily orogastric saline or EGF (50 μg/kg/day). After 3 days, the remnant ileum was removed, apoptotic index (No. apoptotic bodies/crypt) calculated, and expression of mRNA and protein for bax and bcl-w quantified. Results. EGF prevented the expected increase in the apoptotic index after SBR and altered the ratio of bax to bcl-w in favor of cell survival. Conclusion. Following massive small bowel resection, EGF retards rates of enterocyte apoptosis and modifies the expression of bcl-2 family members. By decreasing bax and increasing bcl-w expression, the balance between pro- and anti-apoptotic genes is shifted in favor of cell survival. Alteration of bcl-2 family member expression may be an important mechanism by which EGF reduces the increased enterocyte apoptosis that occurs after massive small bowel resection. (C) 2000 Academic Press.
AB - Background. Following massive small bowel resection (SBR), the expression of bax and bcl-w is associated with increased enterocyte apoptosis. Epidermal growth factor (EGF) has been shown to enhance enterocyte proliferation and retard apoptosis in the adapting bowel. This study examined the effect of EGF on the expression of these bcl-2 family members during adaptation. Materials and methods. Mice (C57Bl/6; n = 38) underwent a 50% SBR or sham operation and then were randomized to receive twice-daily orogastric saline or EGF (50 μg/kg/day). After 3 days, the remnant ileum was removed, apoptotic index (No. apoptotic bodies/crypt) calculated, and expression of mRNA and protein for bax and bcl-w quantified. Results. EGF prevented the expected increase in the apoptotic index after SBR and altered the ratio of bax to bcl-w in favor of cell survival. Conclusion. Following massive small bowel resection, EGF retards rates of enterocyte apoptosis and modifies the expression of bcl-2 family members. By decreasing bax and increasing bcl-w expression, the balance between pro- and anti-apoptotic genes is shifted in favor of cell survival. Alteration of bcl-2 family member expression may be an important mechanism by which EGF reduces the increased enterocyte apoptosis that occurs after massive small bowel resection. (C) 2000 Academic Press.
KW - Apoptosis
KW - Short gut syndrome
KW - bcl-2
UR - http://www.scopus.com/inward/record.url?scp=0034213159&partnerID=8YFLogxK
U2 - 10.1006/jsre.2000.5897
DO - 10.1006/jsre.2000.5897
M3 - Article
C2 - 10816347
AN - SCOPUS:0034213159
VL - 91
SP - 38
EP - 42
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 1
ER -