Enzyme replacement therapy prevents dental defects in a model of hypophosphatasia

M. D. McKee, Y. Nakano, D. L. Masica, J. J. Gray, I. Lemire, R. Heft, M. P. Whyte, P. Crine, J. L. Millán

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

Hypophosphatasia (HPP) occurs from loss-of-function mutation in the tissue-non-specific alkaline phosphatase (TNALP) gene, resulting in extracellular pyrophosphate accumulation that inhibits skeletal and dental mineralization. TNALP-null mice (Akp2-/-) phenocopy human infantile hypophosphatasia; they develop rickets at 1 week of age, and die before being weaned, having severe skeletal and dental hypomineralization and episodes of apnea and vitamin B6-responsive seizures. Delay and defects in dentin mineralization, together with a deficiency in acellular cementum, are characteristic. We report the prevention of these dental abnormalities in Akp2-/- mice receiving treatment from birth with daily injections of a mineral-targeting, human TNALP (sALP-FcD10). sALP-FcD10 prevented hypomineralization of alveolar bone, dentin, and cementum as assessed by micro-computed tomography and histology. Osteopontin - a marker of acellular cementum - was immunolocalized along root surfaces, confirming that acellular cementum, typically missing or reduced in Akp2-/- mice, formed normally. Our findings provide insight concerning how acellular cementum is formed on tooth surfaces to effect periodontal ligament attachment to retain teeth in their osseous alveolar sockets. Furthermore, they provide evidence that this enzyme-replacement therapy, applied early in post-natal life - where the majority of tooth root development occurs, including acellular cementum formation - could prevent the accelerated tooth loss seen in individuals with HPP.

Original languageEnglish
Pages (from-to)470-476
Number of pages7
JournalJournal of Dental Research
Volume90
Issue number4
DOIs
StatePublished - Apr 2011

Keywords

  • cementum
  • dentin
  • osteopontin
  • tissue-non-specific alkaline phosphatase
  • tooth loss

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