Enzyme-linked immunóadsorbent assay for detection of colony-stimulating factor (CSF-1)

Dien Ming B. Chen; Shin Hsu; Hsiu San Lin

doi:10.1016/S0022-1759(83)80024-6

Enzyme-linked immunóadsorbent assay for detection of colony-stimulating factor (CSF-1)

Dien Ming B. Chen, Shin Hsu, Hsiu San Lin

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6 Scopus citations

Abstract

An enzyme-linked immunoadsorbent assay (ELISA) has been developed for the quantitative measurement of L-cell colony-stimulating factor (CSF-1). According to this method, purified antigen (CSF-1) was allowed to adsorb to polystyrene or polyvinyl microtiter wells, which were then incubated with specific rat anti-CSF-1 antiserum with or without standard antigen or unknown test samples. The amount of antibody bound to the solid phase was then quantitated by enzyme (horseradish peroxidase)-conjugated rabbit anti-rat immunoglobulin antibody. This assay has a sensitivity comparable to that of the bioassay (~, 5 U) and can be carried out in a single day.

Original language	English
Pages (from-to)	347-357
Number of pages	11
Journal	Journal of Immunological Methods
Volume	56
Issue number	3
DOIs	https://doi.org/10.1016/S0022-1759(83)80024-6
State	Published - 1983

Keywords

colony
enzyme immunoassay
radioimmunoassay
stimulating factor

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10.1016/S0022-1759(83)80024-6

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title = "Enzyme-linked immun{\'o}adsorbent assay for detection of colony-stimulating factor (CSF-1)",

abstract = "An enzyme-linked immunoadsorbent assay (ELISA) has been developed for the quantitative measurement of L-cell colony-stimulating factor (CSF-1). According to this method, purified antigen (CSF-1) was allowed to adsorb to polystyrene or polyvinyl microtiter wells, which were then incubated with specific rat anti-CSF-1 antiserum with or without standard antigen or unknown test samples. The amount of antibody bound to the solid phase was then quantitated by enzyme (horseradish peroxidase)-conjugated rabbit anti-rat immunoglobulin antibody. This assay has a sensitivity comparable to that of the bioassay (~, 5 U) and can be carried out in a single day.",

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AU - Chen, Dien Ming B.

AU - Hsu, Shin

AU - Lin, Hsiu San

PY - 1983

Y1 - 1983

N2 - An enzyme-linked immunoadsorbent assay (ELISA) has been developed for the quantitative measurement of L-cell colony-stimulating factor (CSF-1). According to this method, purified antigen (CSF-1) was allowed to adsorb to polystyrene or polyvinyl microtiter wells, which were then incubated with specific rat anti-CSF-1 antiserum with or without standard antigen or unknown test samples. The amount of antibody bound to the solid phase was then quantitated by enzyme (horseradish peroxidase)-conjugated rabbit anti-rat immunoglobulin antibody. This assay has a sensitivity comparable to that of the bioassay (~, 5 U) and can be carried out in a single day.

AB - An enzyme-linked immunoadsorbent assay (ELISA) has been developed for the quantitative measurement of L-cell colony-stimulating factor (CSF-1). According to this method, purified antigen (CSF-1) was allowed to adsorb to polystyrene or polyvinyl microtiter wells, which were then incubated with specific rat anti-CSF-1 antiserum with or without standard antigen or unknown test samples. The amount of antibody bound to the solid phase was then quantitated by enzyme (horseradish peroxidase)-conjugated rabbit anti-rat immunoglobulin antibody. This assay has a sensitivity comparable to that of the bioassay (~, 5 U) and can be carried out in a single day.

KW - colony

KW - enzyme immunoassay

KW - radioimmunoassay

KW - stimulating factor

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DO - 10.1016/S0022-1759(83)80024-6

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AN - SCOPUS:0020681450

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3

ER -

Enzyme-linked immunóadsorbent assay for detection of colony-stimulating factor (CSF-1)

Abstract

Keywords

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