Enzyme-linked immunóadsorbent assay for detection of colony-stimulating factor (CSF-1)

Dien Ming B. Chen, Shin Hsu, Hsiu San Lin

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations


    An enzyme-linked immunoadsorbent assay (ELISA) has been developed for the quantitative measurement of L-cell colony-stimulating factor (CSF-1). According to this method, purified antigen (CSF-1) was allowed to adsorb to polystyrene or polyvinyl microtiter wells, which were then incubated with specific rat anti-CSF-1 antiserum with or without standard antigen or unknown test samples. The amount of antibody bound to the solid phase was then quantitated by enzyme (horseradish peroxidase)-conjugated rabbit anti-rat immunoglobulin antibody. This assay has a sensitivity comparable to that of the bioassay (~, 5 U) and can be carried out in a single day.

    Original languageEnglish
    Pages (from-to)347-357
    Number of pages11
    JournalJournal of Immunological Methods
    Issue number3
    StatePublished - 1983


    • colony
    • enzyme immunoassay
    • radioimmunoassay
    • stimulating factor


    Dive into the research topics of 'Enzyme-linked immunóadsorbent assay for detection of colony-stimulating factor (CSF-1)'. Together they form a unique fingerprint.

    Cite this