TY - JOUR
T1 - Enzyme and Cancer Cell Selectivity of Nanoparticles
T2 - Inhibition of 3-D Metastatic Phenotype and Experimental Melanoma by Zinc Oxide
AU - DeLong, Robert K.
AU - Mitchell, Jennifer A.
AU - Tyler Morris, R.
AU - Comer, Jeffrey
AU - Hurst, Miranda N.
AU - Ghosh, Kartik
AU - Wanekaya, Adam
AU - Mudge, Miranda
AU - Schaeffer, Ashley
AU - Washington, Laurie L.
AU - Risor-Marhanka, Azure
AU - Thomas, Spencer
AU - Marroquin, Shanna
AU - Lekey, Amber
AU - Smith, Joshua J.
AU - Garrad, Richard
AU - Aryal, Santosh
AU - Abdelhakiem, Mohamed
AU - Glaspell, Garry P.
N1 - Publisher Copyright:
© 2017 American Scientific Publishers All rights reserved.
PY - 2017/2
Y1 - 2017/2
N2 - Biomedical applications for metal and metal oxide nanoparticles are rapidly increasing. Here their functional impact on two well-characterized model enzymes, Luciferase (Luc) or β-galactosidase (β-Gal) was quantitatively compared. Nickel oxide nanoparticle (NiO-NP) activated β-Gal (>400% control) and boron carbide nanoparticle (B4C-NP) inhibited Luc (<10% control), whereas zinc oxide (ZnO-NP) and cobalt oxide (Co3O4-NP) activated β-Gal to a lesser extent and magnesium oxide (MgO) moderately inhibited both enzymes. Melanoma specific killing was in the order; ZnO > B4C = Cu > MgO > Co3O4 > Fe2O3 > NiO, ZnO-NP inhibiting B16F10 and A375 cells as well as ERK enzyme (>90%) and several other cancer-associated kinases (AKT, CREB, p70S6K). ZnO-NP or nanobelt (NB) serve as photoluminescence (PL) cell labels and inhibit 3-D multi-cellular tumor spheroid (MCTS) growth and were tested in a mouse melanoma model. These results demonstrate nanoparticle and enzyme specific biochemical activity and suggest their utility as new tools to explore the important model metastatic foci 3-D environment and their chemotherapeutic potential.
AB - Biomedical applications for metal and metal oxide nanoparticles are rapidly increasing. Here their functional impact on two well-characterized model enzymes, Luciferase (Luc) or β-galactosidase (β-Gal) was quantitatively compared. Nickel oxide nanoparticle (NiO-NP) activated β-Gal (>400% control) and boron carbide nanoparticle (B4C-NP) inhibited Luc (<10% control), whereas zinc oxide (ZnO-NP) and cobalt oxide (Co3O4-NP) activated β-Gal to a lesser extent and magnesium oxide (MgO) moderately inhibited both enzymes. Melanoma specific killing was in the order; ZnO > B4C = Cu > MgO > Co3O4 > Fe2O3 > NiO, ZnO-NP inhibiting B16F10 and A375 cells as well as ERK enzyme (>90%) and several other cancer-associated kinases (AKT, CREB, p70S6K). ZnO-NP or nanobelt (NB) serve as photoluminescence (PL) cell labels and inhibit 3-D multi-cellular tumor spheroid (MCTS) growth and were tested in a mouse melanoma model. These results demonstrate nanoparticle and enzyme specific biochemical activity and suggest their utility as new tools to explore the important model metastatic foci 3-D environment and their chemotherapeutic potential.
KW - Luciferase (Luc)
KW - Metal Oxide Nanoparticle (MONP)
KW - Multi-Cellular Tumor Spheroids (MCTS)
KW - Nano-Belt (NB)
KW - Photoluminescence (PL)
KW - Two Dimensional Fluorescence Difference Spectroscopy (2-D FDS)
KW - β-Galactosidase (β-Gal)
UR - http://www.scopus.com/inward/record.url?scp=85015980333&partnerID=8YFLogxK
U2 - 10.1166/jbn.2017.2336
DO - 10.1166/jbn.2017.2336
M3 - Article
C2 - 29377653
AN - SCOPUS:85015980333
SN - 1550-7033
VL - 13
SP - 221
EP - 231
JO - Journal of Biomedical Nanotechnology
JF - Journal of Biomedical Nanotechnology
IS - 2
ER -