TY - JOUR
T1 - Enzymatic assay of d-glucuronate using uronate dehydrogenase
AU - Moon, Tae Seok
AU - Yoon, Sang Hwal
AU - Tsang Mui Ching, Mary Jane
AU - Lanza, Amanda M.
AU - Prather, Kristala L.Jones
N1 - Funding Information:
This work was supported by the Office of Naval Research Young Investigator Program (N000140510656) and the National Science Foundation (EEC-0540879). A.M.L. was further supported by a Merck Undergraduate Research Grant (Bioprocess R&D, West Point, PA, USA).
PY - 2009/9/15
Y1 - 2009/9/15
N2 - d-Glucuronate is a key metabolite in the process of detoxification of xenobiotics and in a recently constructed synthetic pathway to produce d-glucaric acid, a "top value-added chemical" from biomass. A simple and specific assay of d-glucuronate would be useful for studying these processes, but existing assays are either time-consuming or nonspecific. Using uronate dehydrogenase cloned from Agrobacterium tumefaciens, we developed an assay for d-glucuronate with a detection limit of 5 μM. This method was shown to be more suitable for a system with many interfering compounds than previous methods and was also applied to assays for myo-inositol oxygenase activity.
AB - d-Glucuronate is a key metabolite in the process of detoxification of xenobiotics and in a recently constructed synthetic pathway to produce d-glucaric acid, a "top value-added chemical" from biomass. A simple and specific assay of d-glucuronate would be useful for studying these processes, but existing assays are either time-consuming or nonspecific. Using uronate dehydrogenase cloned from Agrobacterium tumefaciens, we developed an assay for d-glucuronate with a detection limit of 5 μM. This method was shown to be more suitable for a system with many interfering compounds than previous methods and was also applied to assays for myo-inositol oxygenase activity.
UR - http://www.scopus.com/inward/record.url?scp=67650474334&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.05.032
DO - 10.1016/j.ab.2009.05.032
M3 - Article
C2 - 19481054
AN - SCOPUS:67650474334
SN - 0003-2697
VL - 392
SP - 183
EP - 185
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -