Entry of Plasmodium berghei sporozoites into cultured cells, and their transformation into trophozoites

M. R. Hollingdale, P. Leland, J. L. Leef, A. L. Schwartz

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The attachment and entry of Plasmodium berghei sporozoites to cultured human lung fibroblast (WI38) or hepatoma (HepG2-A16) cells in vitro has been visualized using an immunoperoxidase technique coupled with light microscopy. Attachment and entry was substantially more frequent with HepG2-A16 cells, and appeared to be mediated by the Pb44 sporozoite surface protective antigen. When sporozoites were incubated with intact monoclonal antibodies to Pb44 or their monovalent Fab fragments, attachment was inhibited, suggesting that this technique may be an in vitro assay of protective immunity. Sporozoites appeared to enter cells actively, rather than by cell phagocytosis. Once within a membrane-bound vacuole, the sporozoite transformed into a trophozoite. It was suggested that Pb44 recognized a specific cell receptor, and that this technique may permit receptor characterization.

Original languageEnglish
Pages (from-to)685-690
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume32
Issue number4
DOIs
StatePublished - 1983

Fingerprint

Dive into the research topics of 'Entry of Plasmodium berghei sporozoites into cultured cells, and their transformation into trophozoites'. Together they form a unique fingerprint.

Cite this