Enterobacteriaceae Act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis

Wendy S. Garrett, Carey A. Gallini, Tanya Yatsunenko, Monia Michaud, Andrea Dubois, Mary L. Delaney, Shivesh Punit, Maria Karlsson, Lynn Bry, Jonathan N. Glickman, Jeffrey I. Gordon, Andrew B. Onderdonk, Laurie H. Glimcher

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474 Scopus citations

Abstract

Disruption of homeostasis between the host immune system and the intestinal microbiota leads to inflammatory bowel disease (IBD). Whether IBD is instigated by individual species or disruptions of entire microbial communities remains controversial. We characterized the fecal microbial communities in the recently described T-bet-/- × Rag2-/- ulcerative colitis (TRUC) model driven by T-bet deficiency in the innate immune system. 16S rRNA-based analysis of TRUC and Rag2-/- mice revealed distinctive communities that correlate with host genotype. The presence of Klebsiella pneumoniae and Proteus mirabilis correlates with colitis in TRUC animals, and these TRUC-derived strains can elicit colitis in Rag2-/- and WT adults but require a maternally transmitted endogenous microbial community for maximal intestinal inflammation. Cross-fostering experiments indicated a role for these organisms in maternal transmission of disease. Our findings illustrate how gut microbial communities work in concert with specific culturable colitogenic agents to cause IBD.

Original languageEnglish
Pages (from-to)292-300
Number of pages9
JournalCell Host and Microbe
Volume8
Issue number3
DOIs
StatePublished - Sep 16 2010

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    Garrett, W. S., Gallini, C. A., Yatsunenko, T., Michaud, M., Dubois, A., Delaney, M. L., Punit, S., Karlsson, M., Bry, L., Glickman, J. N., Gordon, J. I., Onderdonk, A. B., & Glimcher, L. H. (2010). Enterobacteriaceae Act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis. Cell Host and Microbe, 8(3), 292-300. https://doi.org/10.1016/j.chom.2010.08.004