TY - JOUR
T1 - Enterically induced regulation of systemic immune responses. II. Suppression of proliferating T cells by an Lyt-1+,2- T effector cell
AU - Silverman, G. A.
AU - Peri, B. A.
AU - Fitch, F. W.
AU - Rothberg, R. M.
PY - 1983
Y1 - 1983
N2 - Peripheral lymph node cells from C3H mice that were fed and injected with bovine serum albumin (REG cells) demonstrate an impaired proliferative response to antigenic stimulation in vitro compared to cells from mice only injected with BSA. To determine whether suppressor cells contributed to this enterically induced impairment of systemic T cell responses, REG cells were pretreated with various monoclonal antibodies and complement (C), and were then co-cultured with antigen-reactive indicator T cells (IND) from parenterally immunized mice. Proliferation of IND cells ([3H]thymidine uptake) was suppressed only if REG cells were treated with anti-Lyt-2 and C before co-culture. The ability of anti-Lyt-1 plus anti-Lyt-2 and C treatment to abrogate suppression suggested that the suppressor effect was due to an Lyt-1+,2- REG cell. Suppression was independent of Lyt-2+ IND cells, and was observed at different antigen concentrations, cultivation times, and cell densities. The cells responsible for suppressor activity were radiosensitive, nylon wool non-adhernt, and antigen specific. These data suggest that are Lyt-1+,2- T cell could be an important component in mediating enterically induced regulation of systemic T cell responses.
AB - Peripheral lymph node cells from C3H mice that were fed and injected with bovine serum albumin (REG cells) demonstrate an impaired proliferative response to antigenic stimulation in vitro compared to cells from mice only injected with BSA. To determine whether suppressor cells contributed to this enterically induced impairment of systemic T cell responses, REG cells were pretreated with various monoclonal antibodies and complement (C), and were then co-cultured with antigen-reactive indicator T cells (IND) from parenterally immunized mice. Proliferation of IND cells ([3H]thymidine uptake) was suppressed only if REG cells were treated with anti-Lyt-2 and C before co-culture. The ability of anti-Lyt-1 plus anti-Lyt-2 and C treatment to abrogate suppression suggested that the suppressor effect was due to an Lyt-1+,2- REG cell. Suppression was independent of Lyt-2+ IND cells, and was observed at different antigen concentrations, cultivation times, and cell densities. The cells responsible for suppressor activity were radiosensitive, nylon wool non-adhernt, and antigen specific. These data suggest that are Lyt-1+,2- T cell could be an important component in mediating enterically induced regulation of systemic T cell responses.
UR - http://www.scopus.com/inward/record.url?scp=0021012446&partnerID=8YFLogxK
M3 - Article
C2 - 6196395
AN - SCOPUS:0021012446
VL - 131
SP - 2656
EP - 2661
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -