TY - JOUR
T1 - Enhancer-facilitated expression of a human H4 histone gene
AU - Shiels, Alan
AU - Marashi, Farhad
AU - Stein, Gary
AU - Stein, Janet
N1 - Funding Information:
We are indebted to Drs. Dan Greenspan and Sherman Weissman for providing plasmid pDGOI4 which contained the SV40 enhancer region. This work was funded by grants awarded by the National Institutes of Health (GM32010), the National Science Foundation (PCM 83 18075) and the March Dimes Birth Defects Foundation (i-813).
PY - 1985/2/28
Y1 - 1985/2/28
N2 - Cultured mammalian cells were transfected with a recombinant human H4 histone gene. S1 nuclease mapping of cellular RNAs from transfected cells revealed: (i) correct initiation of transcription at the cap site, with some transcripts originating from other sites in the 5′ flanking region of this H4 gene; (ii) cis-linkage of an SV-40 transcriptional enhancer element upstream of the H4 5′-flanking region resulted in about a 50-fold increase in the level of correctly initiated H4 mRNA and (iii) in a heterologous murine system stability of human H4 mRNAs was apparently sensitive to inhibition of DNA-synthesis by hydroxyurea. Our results suggest that certain sequences required for the initiation of a human H4 histone gene transcript reside within the 210 nucleotides immediately upstream from the cap site and that the level of expression is influenced by the introduction of an enhancer element.
AB - Cultured mammalian cells were transfected with a recombinant human H4 histone gene. S1 nuclease mapping of cellular RNAs from transfected cells revealed: (i) correct initiation of transcription at the cap site, with some transcripts originating from other sites in the 5′ flanking region of this H4 gene; (ii) cis-linkage of an SV-40 transcriptional enhancer element upstream of the H4 5′-flanking region resulted in about a 50-fold increase in the level of correctly initiated H4 mRNA and (iii) in a heterologous murine system stability of human H4 mRNAs was apparently sensitive to inhibition of DNA-synthesis by hydroxyurea. Our results suggest that certain sequences required for the initiation of a human H4 histone gene transcript reside within the 210 nucleotides immediately upstream from the cap site and that the level of expression is influenced by the introduction of an enhancer element.
UR - http://www.scopus.com/inward/record.url?scp=0022002048&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(85)80150-9
DO - 10.1016/S0006-291X(85)80150-9
M3 - Article
C2 - 2983708
AN - SCOPUS:0022002048
SN - 0006-291X
VL - 127
SP - 239
EP - 246
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -