TY - JOUR
T1 - Enhancement of hippocampal excitatory synaptic transmission by platelet-activating factor
AU - Clark, Gary D.
AU - Happel, Leo T.
AU - Zorumski, Charles F.
AU - Bazan, Nicolas G.
PY - 1992/12
Y1 - 1992/12
N2 - The biologically active lipid platelet-activating factor (1-O-alkyl-2-acetykn-glycero-3-phosphorylcholine, PAF) is a mediator of inflammatory and immune responses, and it accumulates in the brain during convulsions or ischemia. We have examined whether PAF may play a second messenger role in the central nervous system by studying effects on synaptic transmission in cultured hippocampal neurons. Carbamyl-PAF, a nonhydrolyzable PAF analog with a similar pharmacologic profile, augmented glutamate-mediated, evoked excitatory synaptic transmission and increased the frequency of spontaneous miniature excitatory synaptic events without increasing their amplitude or altering their time course. This compound had no significant effect on γ-aminobutyric acid-mediated inhibitory synaptic responses. Lyso-PAF, the biologically inactive metabolic intermediate, had no effect on synaptic transmission. Moreover, the enhancement of excitatory synaptic transmission by carbamyl-PAF was blocked by a PAF receptor antagonist. These results indicate a specific presynaptic effect of PAF in enhancing excitatory synaptic transmission in cultured rat hippocampal neurons.
AB - The biologically active lipid platelet-activating factor (1-O-alkyl-2-acetykn-glycero-3-phosphorylcholine, PAF) is a mediator of inflammatory and immune responses, and it accumulates in the brain during convulsions or ischemia. We have examined whether PAF may play a second messenger role in the central nervous system by studying effects on synaptic transmission in cultured hippocampal neurons. Carbamyl-PAF, a nonhydrolyzable PAF analog with a similar pharmacologic profile, augmented glutamate-mediated, evoked excitatory synaptic transmission and increased the frequency of spontaneous miniature excitatory synaptic events without increasing their amplitude or altering their time course. This compound had no significant effect on γ-aminobutyric acid-mediated inhibitory synaptic responses. Lyso-PAF, the biologically inactive metabolic intermediate, had no effect on synaptic transmission. Moreover, the enhancement of excitatory synaptic transmission by carbamyl-PAF was blocked by a PAF receptor antagonist. These results indicate a specific presynaptic effect of PAF in enhancing excitatory synaptic transmission in cultured rat hippocampal neurons.
UR - http://www.scopus.com/inward/record.url?scp=0027081205&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(92)90078-R
DO - 10.1016/0896-6273(92)90078-R
M3 - Article
C2 - 1334422
AN - SCOPUS:0027081205
VL - 9
SP - 1211
EP - 1216
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 6
ER -