Enhancement of a label-free dielectrophoretic cell sorter with an integrated impedance detection system

M. Carminati, M. D. Vahey, A. Rottigni, G. Ferrari, J. Voldman, M. Sampietro

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

8 Scopus citations

Abstract

We present a miniaturized CMOS impedance detector integrated within a dielectrophoretic microfluidic device for label-free separation, enabling the sorting and counting of particle suspensions in a fully electrical manner. This approach makes possible a truly portable system for cell sorting and analysis. Towards this end, the design of ad hoc co-planar electrodes for impedance sensing, supported by numerical simulations, is illustrated. The validation of the system with 10μm polystyrene beads is reported. A USB-controlled single-chip lock-in demodulator allows high-throughput counting of resistive peaks of 1% amplitude, with a signal-to-noise ratio >10 and with a time resolution of 200μs.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1394-1396
Number of pages3
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Flow Cytometry
  • Intrinsic Cell Separation
  • Lock-In Impedance Detection

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