TY - GEN
T1 - Enhancement of a label-free dielectrophoretic cell sorter with an integrated impedance detection system
AU - Carminati, M.
AU - Vahey, M. D.
AU - Rottigni, A.
AU - Ferrari, G.
AU - Voldman, J.
AU - Sampietro, M.
PY - 2010
Y1 - 2010
N2 - We present a miniaturized CMOS impedance detector integrated within a dielectrophoretic microfluidic device for label-free separation, enabling the sorting and counting of particle suspensions in a fully electrical manner. This approach makes possible a truly portable system for cell sorting and analysis. Towards this end, the design of ad hoc co-planar electrodes for impedance sensing, supported by numerical simulations, is illustrated. The validation of the system with 10μm polystyrene beads is reported. A USB-controlled single-chip lock-in demodulator allows high-throughput counting of resistive peaks of 1% amplitude, with a signal-to-noise ratio >10 and with a time resolution of 200μs.
AB - We present a miniaturized CMOS impedance detector integrated within a dielectrophoretic microfluidic device for label-free separation, enabling the sorting and counting of particle suspensions in a fully electrical manner. This approach makes possible a truly portable system for cell sorting and analysis. Towards this end, the design of ad hoc co-planar electrodes for impedance sensing, supported by numerical simulations, is illustrated. The validation of the system with 10μm polystyrene beads is reported. A USB-controlled single-chip lock-in demodulator allows high-throughput counting of resistive peaks of 1% amplitude, with a signal-to-noise ratio >10 and with a time resolution of 200μs.
KW - Flow Cytometry
KW - Intrinsic Cell Separation
KW - Lock-In Impedance Detection
UR - http://www.scopus.com/inward/record.url?scp=79958026314&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:79958026314
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1394
EP - 1396
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -