Abstract
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.
Original language | English |
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Pages (from-to) | 220-222 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 156 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1986 |
Keywords
- 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride
- N-hydroxysulfosuccinimide
- carbodiimide
- coupling
- peptides
- proteins