Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

James V. Staros; Rick W. Wright; Deborah M. Swingle

doi:10.1016/0003-2697(86)90176-4

Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

James V. Staros, Rick W. Wright, Deborah M. Swingle

Department of Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

788 Scopus citations

Abstract

Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.

Original language	English
Pages (from-to)	220-222
Number of pages	3
Journal	Analytical Biochemistry
Volume	156
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(86)90176-4
State	Published - Jul 1986

Keywords

1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride
N-hydroxysulfosuccinimide
carbodiimide
coupling
peptides
proteins

Access to Document

10.1016/0003-2697(86)90176-4

Cite this

@article{6f5b8747b9f444c3869feccabbea59bd,

title = "Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions",

abstract = "Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.",

keywords = "1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, N-hydroxysulfosuccinimide, carbodiimide, coupling, peptides, proteins",

author = "Staros, {James V.} and Wright, {Rick W.} and Swingle, {Deborah M.}",

note = "Funding Information: {\textquoteright} This work was supported by research grants from the National Institutes of Health (AM31880 and AM25489). R.W.R. was a participant in the Summer Student Research Program in Diabetes at Vanderbilt, supported by NIH Training Grant T35 AM07383.",

year = "1986",

month = jul,

doi = "10.1016/0003-2697(86)90176-4",

language = "English",

volume = "156",

pages = "220--222",

journal = "Analytical Biochemistry",

issn = "0003-2697",

number = "1",

}

TY - JOUR

T1 - Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

AU - Staros, James V.

AU - Wright, Rick W.

AU - Swingle, Deborah M.

N1 - Funding Information: ’ This work was supported by research grants from the National Institutes of Health (AM31880 and AM25489). R.W.R. was a participant in the Summer Student Research Program in Diabetes at Vanderbilt, supported by NIH Training Grant T35 AM07383.

PY - 1986/7

Y1 - 1986/7

N2 - Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.

AB - Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.

KW - 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride

KW - N-hydroxysulfosuccinimide

KW - carbodiimide

KW - coupling

KW - peptides

KW - proteins

UR - http://www.scopus.com/inward/record.url?scp=0022512416&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(86)90176-4

DO - 10.1016/0003-2697(86)90176-4

M3 - Article

C2 - 3740412

AN - SCOPUS:0022512416

VL - 156

SP - 220

EP - 222

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -

Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this