Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

James V. Staros, Rick W. Wright, Deborah M. Swingle

Research output: Contribution to journalArticle

721 Scopus citations

Abstract

Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.

Original languageEnglish
Pages (from-to)220-222
Number of pages3
JournalAnalytical Biochemistry
Volume156
Issue number1
DOIs
StatePublished - Jul 1986

Keywords

  • 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride
  • N-hydroxysulfosuccinimide
  • carbodiimide
  • coupling
  • peptides
  • proteins

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