Abstract
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.
| Original language | English |
|---|---|
| Pages (from-to) | 220-222 |
| Number of pages | 3 |
| Journal | Analytical Biochemistry |
| Volume | 156 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1986 |
Keywords
- 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride
- N-hydroxysulfosuccinimide
- carbodiimide
- coupling
- peptides
- proteins
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Cite this
Staros, J. V., Wright, R. W., & Swingle, D. M. (1986). Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions. Analytical Biochemistry, 156(1), 220-222. https://doi.org/10.1016/0003-2697(86)90176-4