Enhanced Synthesis of Leukotriene B4 by Colonic Mucosa in Inflammatory Bowel Disease

Pinchas Sharon, William F. Stenson

Research output: Contribution to journalArticlepeer-review

657 Scopus citations

Abstract

Leukotriene B4, an arachidonic acid metabolite, is a potent chemotactic agent, and is thought to be an important mediator of inflammation. To investigate the role of this compound as a mediator of inflammation in inflammatory bowel disease, arachidonic acid was incubated with ionophore and colonic mucosa from patients with inflammatory bowel disease and from normal subjects. Mucosa from patients with inflammatory bowel disease converted 2.17% of exogenous arachidonate to leukotriene B4; mucosa from normal subjects converted 0.37%. The production of leukotriene was blocked by sulfasalazine. To determine if inflammatory bowel mucosa contained endogenous leukotriene B4, lipid extracts were analyzed by high pressure liquid chromatography. Mucosa from patients with inflammatory bowel disease contained 254 ng of leukotriene B4 per gram and mucosa from normal subjects contained < 5 ng of leukotriene B4 per gram. The presence of significant amounts of leukotriene B4 in colonic mucosa in patients with inflammatory bowel disease, combined with the known biologic effects of leukotriene B4, suggests that it may be an important mediator of inflammation in inflammatory bowel disease.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalGastroenterology
Volume86
Issue number3
DOIs
StatePublished - 1984

Keywords

  • 12-HETE
  • 12-hydroxy-5,8,10,14-eicosatetraenoic acid
  • 15-HETE
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid
  • 5-HETE
  • 5-hydroxy-6,8,11,14-eicosatetraenoic acid
  • 5S, 128-diHETE
  • 5S, 12S dihydroxy (E,Z,E,Z) 6,8,10,14eicosatetraenoic acid
  • HPLC
  • IBD
  • LTB
  • TLC
  • TxB
  • high pressure liquid chromatography
  • inflammatory bowel disease
  • leukotriene B
  • thin layer chromatography
  • thromboxane B

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