Enhanced neurite growth from mammalian neurons in three-dimensional salmon fibrin gels

Yo El Ju, Paul A. Janmey, Margaret E. McCormick, Evelyn S. Sawyer, Lisa A. Flanagan

Research output: Contribution to journalArticlepeer-review

83 Scopus citations


Three-dimensional fibrin matrices have been used as cellular substrates in vitro and as bridging materials for central nervous system repair. Cells can be embedded within fibrin gels since the polymerization process is non-toxic, making fibrin an attractive scaffold for transplanted cells. Most studies have utilized fibrin prepared from human or bovine blood proteins. However, fish fibrin may be well suited for neuronal growth since fish undergo remarkable central nervous system regeneration and molecules implicated in this process are present in fibrin. We assessed the growth of mammalian central nervous system neurons in bovine, human, and salmon fibrin and found that salmon fibrin gels encouraged the greatest degree of neurite (dendrite and axon) growth and were the most resistant to degradation by cellular proteases. The neurite growth-promoting effect was not due to the thrombin used to polymerize the gels nor to any copurifying plasminogen. Copurified fibronectin partially accounted for the effect on neurites, and blockade of fibrinogen/fibrin-binding integrins markedly decreased neurite growth. Anion exchange chromatography revealed different elution profiles for salmon and mammalian fibrinogens. These data demonstrate that salmon fibrin encourages the growth of neurites from mammalian neurons and suggest that salmon fibrin may be a beneficial scaffold for neuronal regrowth after CNS injury.

Original languageEnglish
Pages (from-to)2097-2108
Number of pages12
Issue number12
StatePublished - Apr 2007


  • Cortical
  • Integrin
  • Matrix
  • Nerve regeneration
  • Scaffold
  • Spinal cord


Dive into the research topics of 'Enhanced neurite growth from mammalian neurons in three-dimensional salmon fibrin gels'. Together they form a unique fingerprint.

Cite this