Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells

Sun Kyung Kim; Cheol Heui Yun; Seung Hyun Han

doi:10.1016/j.canlet.2013.02.038

Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells

Sun Kyung Kim
, Cheol Heui Yun
, Seung Hyun Han

Division of Immunobiology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ-irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.

Original language	English
Pages (from-to)	278-288
Number of pages	11
Journal	Cancer Letters
Volume	335
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2013.02.038
State	Published - Jul 28 2013

Keywords

γ-Irradiation
Cancer cell-killing
Cytotoxic molecules
Dendritic cells
HT-29

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10.1016/j.canlet.2013.02.038

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title = "Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells",

abstract = "Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ-irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.",

keywords = "γ-Irradiation, Cancer cell-killing, Cytotoxic molecules, Dendritic cells, HT-29",

author = "Kim, \{Sun Kyung\} and Yun, \{Cheol Heui\} and Han, \{Seung Hyun\}",

note = "Funding Information: This work was supported by grants from the Expansion of Nuclear R\&D Infrastructure Program through the Korea Science and Engineering Foundation ( 2008-01571 ), the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology ( 2012-0008693 and 2012-0000492 ), and the R\&D Convergence Center Support Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea .",

year = "2013",

month = jul,

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doi = "10.1016/j.canlet.2013.02.038",

language = "English",

volume = "335",

pages = "278--288",

journal = "Cancer Letters",

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T1 - Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells

AU - Kim, Sun Kyung

AU - Yun, Cheol Heui

AU - Han, Seung Hyun

N1 - Funding Information: This work was supported by grants from the Expansion of Nuclear R&D Infrastructure Program through the Korea Science and Engineering Foundation ( 2008-01571 ), the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology ( 2012-0008693 and 2012-0000492 ), and the R&D Convergence Center Support Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea .

PY - 2013/7/28

Y1 - 2013/7/28

N2 - Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ-irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.

AB - Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ-irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.

KW - γ-Irradiation

KW - Cancer cell-killing

KW - Cytotoxic molecules

KW - Dendritic cells

KW - HT-29

UR - https://www.scopus.com/pages/publications/84878218095

U2 - 10.1016/j.canlet.2013.02.038

DO - 10.1016/j.canlet.2013.02.038

M3 - Article

C2 - 23485725

AN - SCOPUS:84878218095

SN - 0304-3835

VL - 335

SP - 278

EP - 288

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells

Abstract

Keywords

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