Abstract
Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ-irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.
Original language | English |
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Pages (from-to) | 278-288 |
Number of pages | 11 |
Journal | Cancer Letters |
Volume | 335 |
Issue number | 2 |
DOIs | |
State | Published - Jul 28 2013 |
Keywords
- γ-Irradiation
- Cancer cell-killing
- Cytotoxic molecules
- Dendritic cells
- HT-29