68 Scopus citations

Abstract

We compared lipoxygenase activities of lung macrophages obtained from bronchoalveolar lavage to activities of blood monocytes purified by using discontinuous plasma/Percoll density gradients and adherence to tissue culture plastic in five normal subjects. Cells were incubated with ionophore A23187 (10-9 to 10-5 M) or arachidonic acid (0.12 to 80 μM) for 1 to 60 min at 37°C to construct dose-response and time-dependent curves of lipoxygenase product generation. Products were identified and were quantified by using high-pressure liquid chromatography and ultraviolet spectroscopy. Under all conditions of product generation, both macrophages and monocytes generated predominantly (5S-12R)-dihydroxy-(6Z,8E,10E,14Z)-eicosatetraenoic acid (leukotriene B4 (LTB4)) and (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoic acid (5-HETE), but, in each subject, macrophages invariably released greater amounts of LTB4 and 5-HETE than monocytes. In response to A23187, macrophages released a maximum of 183 ± 96 pmol of LTB4 and 168 ± 108 pmol of 5-HETE per 106 cells (mean ± SEM), whereas monocytes released only 16 ± 1 and 18 ± 8 pmol per 106 cells of LTB4 and 5-HETE, respectively. After adding arachidonic acid, macrophages released a maximum of 52 ± 21 pmol of LTB4 and 223 ± 66 pmol of 5-HETE, whereas monocytes released no detectable products. The results suggest that mononuclear phagocyte maturation in the lung may be accompanied by an enhanced ability to generate 5-lipoxygenase products.

Original languageEnglish
Pages (from-to)1546-1550
Number of pages5
JournalJournal of Immunology
Volume138
Issue number5
StatePublished - 1987

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