Engineering recombinant orsay virus directly in the metazoan host Caenorhabditis elegans

Hongbing Jiang, Carl J. Franz, David Wang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The recent identification of Orsay virus, the first virus that is capable of naturally infecting Caenorhabditis elegans, provides a unique opportunity to explore host-virus interaction studies in this invaluable model organism. A key feature of this system is the robust genetic tractability of the host, C. elegans, which would ideally be complemented by the ability to genetically manipulate Orsay virus in parallel. To this end, we developed a plasmid-based reverse genetics system for Orsay virus by creating transgenic C. elegans strains harboring Orsay virus cDNAs. Both wild-type and mutant Orsay viruses, including a FLAG epitopetagged recombinant Orsay virus, were generated by use of the reverse genetics system. This is the first plasmid-based virus reverse genetics system in the metazoan C. elegans. The Orsay virus reverse genetics we established will serve as a fundamental tool in host-virus interaction studies in the model organism C. elegans.

Original languageEnglish
Pages (from-to)11774-11781
Number of pages8
JournalJournal of virology
Volume88
Issue number20
DOIs
StatePublished - 2014

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