TY - JOUR
T1 - Engineering microbial diagnostics and therapeutics with smart control
AU - Amrofell, Matthew B.
AU - Rottinghaus, Austin G.
AU - Moon, Tae Seok
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/12
Y1 - 2020/12
N2 - Microbes have become an increasingly powerful chassis for developing diagnostic and therapeutic technologies. While many of the earlier engineering efforts used microbes that expressed relevant proteins constitutively, more microbes are being engineered to express them with region-selectivity and disease-responsiveness through biosensors. Such ‘smart’ microbes have been developed to diagnose and treat a wide range of disorders and diseases, including bacterial infections, cancers, inflammatory disorders, and metabolic disorders. In this review, we discuss synthetic biology technologies that have been applied to engineer microbes for biomedical applications, focusing on recent reports that demonstrate microbial sensing by using animal models or clinical samples. Advances in synthetic biology will enable engineered microbes to significantly improve the medical field.
AB - Microbes have become an increasingly powerful chassis for developing diagnostic and therapeutic technologies. While many of the earlier engineering efforts used microbes that expressed relevant proteins constitutively, more microbes are being engineered to express them with region-selectivity and disease-responsiveness through biosensors. Such ‘smart’ microbes have been developed to diagnose and treat a wide range of disorders and diseases, including bacterial infections, cancers, inflammatory disorders, and metabolic disorders. In this review, we discuss synthetic biology technologies that have been applied to engineer microbes for biomedical applications, focusing on recent reports that demonstrate microbial sensing by using animal models or clinical samples. Advances in synthetic biology will enable engineered microbes to significantly improve the medical field.
UR - http://www.scopus.com/inward/record.url?scp=85086594321&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2020.05.006
DO - 10.1016/j.copbio.2020.05.006
M3 - Review article
C2 - 32563763
AN - SCOPUS:85086594321
SN - 0958-1669
VL - 66
SP - 11
EP - 17
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -