Enforced expression of EBF in hematopoietic stem cells restricts lymphopoiesis to the B cell lineage

Zheng Zhang, Claudiu V. Cotta, Robert P. Stephan, Cristina G. De Guzman, Christopher A. Klug

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Mice deficient in early B cell factor (EBF) are blocked at the progenitor B cell stage prior to immunoglobulin gene rearrangement. The EBF-dependent block in B cell development occurs near the onset of B-lineage commitment, which raises the possibility that EBF may act instructively to specify the B cell fate from uncommitted, multipotential progenitor cells. To test this hypothesis, we transduced enriched hematopoietic progenitor cells with a retroviral vector that coexpressed EBF and the green fluorescent protein (GFP). Mice reconstituted with EBF-expressing cells showed a near complete absence of T lymphocytes. Spleen and peripheral blood samples were >95 and 90% GFP +EBF+ mature B cells, respectively. Both NK and lymphoid-derived dendritic cells were also significantly reduced compared with control-transplanted mice. These data suggest that EBF can restrict lymphopoiesis to the B cell lineage by blocking development of other lymphoid-derived cell pathways.

Original languageEnglish
Pages (from-to)4759-4769
Number of pages11
JournalEMBO Journal
Volume22
Issue number18
DOIs
StatePublished - Sep 15 2003

Keywords

  • B cell development
  • EBF
  • Hematopoietic stem cell
  • T cell development
  • Transcription factor

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