The energy metabolism of hypothermically preserved rat livers was studied. For this purpose, the organs were preserved by in situ flushing and cold storage. Biopsies were performed immediately after flushing and after storage for 3h, 6h, 12h, 24h and 36h in UW solution and Ringer's at 4°C. Biopsies were then analyzed for adenine nucleotides using high-performance liquid chromatography. In both solutions, concentrations of ATP and ADP as well as the energy charge decreased substantially while AMP concentrations increased within the first hours of preservation. In UW solution, the total adenine nucleotide pool (TAN) was found to remain approximately constant during 24h of cold ischemia, whereas in Ringer's it decreased after 12 h. Thus, significantly higher concentrations of AMP and TAN were measured for UW solution after 24h and 36h of cold storage. In conclusion, UW solution extends the delay of adenine nucleotide degradation up to 24h of preservation.
|Number of pages||4|
|Journal||Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft|
|State||Published - Jan 1 1994|