@article{ce3d3d5202624e12bd8cc49ebc84b396,
title = "Endosymbiotic adaptations in three new bacterial species associated with Dictyostelium discoideum: Paraburkholderia agricolaris sp. nov., Paraburkholderia hayleyella sp. nov., and Paraburkholderia bonniea sp. nov",
abstract = "Here we give names to three new species of Paraburkholderia that can remain in symbiosis indefinitely in the spores of a soil dwelling eukaryote, Dictyostelium discoideum. The new species P. agricolaris sp. nov., P. hayleyella sp. nov., and P. bonniea sp. nov. are widespread across the eastern USA and were isolated as internal symbionts of wild-collected D. discoideum. We describe these sp. nov. using several approaches. Evidence that they are each a distinct new species comes from their phylogenetic position, average nucleotide identity, genome-genome distance, carbon usage, reduced length, cooler optimal growth temperature, metabolic tests, and their previously described ability to invade D. discoideum amoebae and form a symbiotic relationship. All three of these new species facilitate the prolonged carriage of food bacteria by D. discoideum, though they themselves are not food. Further studies of the interactions of these three new species with D. discoideum should be fruitful for understanding the ecology and evolution of symbioses.",
keywords = "Dictyostelium, Mutualism, Paraburkholderia, Social amoebae, Symbiosis",
author = "Brock, {Debra A.} and Suegene Noh and Hubert, {Alicia N.M.} and Haselkorn, {Tamara S.} and Susanne DiSalvo and Suess, {Melanie K.} and Bradley, {Alexander S.} and Mahboubeh Tavakoli-Nezhad and Geist, {Katherine S.} and Queller, {David C.} and Strassmann, {Joan E.}",
note = "Funding Information: The following grant information was disclosed by the authors: National Science Foundation: NSF IOS-1656756, NSF DEB-1753743. John Templeton Foundation: 43667. Funding Information: We thank the Queller/Strassmann lab group for much useful advice and help, particularly Tyler Larsen and Trey Scott. We specifically thank Jason Zuke for help with microscopy and Joe LaManna for help with principal component analysis in R. We are grateful to James Tiedje at Michigan State University for Paraburkholderia xenovorans LB400 and to Dr. Lionel Moulin at IPME, University of Montpellier, France for Paraburkholderia phymatum STM 815. This material is based on work supported by the National Science Foundation under grant number NSF IOS-1656756, NSF DEB-1753743 and the John Templeton Foundation grant no. 43667. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The following grant information was disclosed by the authors: National Science Foundation: NSF IOS-1656756, NSF DEB-1753743. John Templeton Foundation: 43667. Funding Information: This material is based on work supported by the National Science Foundation under grant number NSF IOS-1656756, NSF DEB-1753743 and the John Templeton Foundation grant no. 43667. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2020 Brock et al.",
year = "2020",
doi = "10.7717/peerj.9151",
language = "English",
volume = "2020",
journal = "PeerJ",
issn = "2167-8359",
number = "5",
}