Endogenous IL-1 is required for neutrophil recruitment and macrophage activation during murine listeriosis

Howard W. Rogers, Catherine S. Tripp, Robert D. Schreiber, Emil R. Unanue

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69 Scopus citations

Abstract

By using a mixture of neutralizing mAbs to IL-1α, IL-1β, and to the type I IL-1R, we previously documented a regulatory role for IL-1 in the development of anti-Listeria responses in mice. Both normal C.B-17 and severe combined immunodeficiency (SCID) mice injected with this mixture of Abs exhibit decreased resistance to Listeria. In this study, we demonstrate that the neutralization of IL-1 activity in SCID mice results in a major defect in neutrophil migration to the peritoneum, in response to Listeria infection. Moreover, anti-IL-1 treatment also inhibits Listeria-induced peripheral blood leukocytosis at all time points examined. We also show that mice injected with anti-IL-1 Abs failed to elaborate class II MHC-positive peritoneal macrophages in vivo at any time during Listeria infection. Even though peritoneal macrophages from anti-IL-1-treated Listeria-infected mice are not activated to express MHC class II molecules, IFN-γ production in vivo is normal. Moreover, the macrophages are unresponsive to IFN-γ in vitro, as assayed by MHC class II expression, even when rIL-1 is added. rIL-1 also is unable to increase the expression of IFN-γ-induced surface class II MHC molecules on resident peritoneal macrophages in vitro. These results indicate that endogenously produced IL-1 plays an important role in Listeria-dependent neutrophil migration, increase in blood leukocyte number, generation of MHC class II-positive macrophages in vivo, and macrophage responsiveness to IFN- γ.

Original languageEnglish
Pages (from-to)2093-2101
Number of pages9
JournalJournal of Immunology
Volume153
Issue number5
StatePublished - Sep 1 1994

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