Emergence of Klebsiella pneumoniae co-producing NDM-1, OXA-48, CTX-M-15, CMY-16, QnrA and ArmA in Switzerland

Salome N. Seiffert, Jonas Marschall, Vincent Perreten, Alessandra Carattoli, Hansjakob Furrer, Andrea Endimiani

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Extensively drug-resistant (XDR) Klebsiella pneumoniae isolates usually carry a single carbapenemase (e.g. KPC, NDM, OXA-48-like). Here we describe an XDR K. pneumoniae of sequence type 101 that was detected in the screening rectal swab of a patient transferred from the intensive care unit of a hospital located in Belgrade (Serbia) to Bern University Hospital (Switzerland). The isolate was resistant to all antibiotics with the exception of colistin [minimum inhibitory concentration] (MIC ≤ 0.125 μg/mL), tigecycline (MIC = 0.5 μg/mL) and fosfomycin (MIC = 2 μg/mL). The isolate co-possessed class B (NDM-1) and class D (OXA-48) carbapenemases, class A extended-spectrum β-lactamase (CTX-M-15), class C cephalosporinase (CMY-16), ArmA 16S rRNA methyltransferase, substitutions in GyrA and ParC, loss of OmpK35 porin, as well as other genes conferring resistance to quinolones (qnrA), tetracyclines [tet(A)], sulfonamides (sul1, sul2), trimethoprim (dfrA12, dfrA14), rifampicin (arr-1), chloramphenicol (cmlA1, floR) and streptomycin (aadA1). The patient was placed under contact isolation precautions preventing the spread of this nearly untreatable pathogen.

Original languageEnglish
Pages (from-to)260-262
Number of pages3
JournalInternational Journal of Antimicrobial Agents
Volume44
Issue number3
DOIs
StatePublished - Sep 1 2014

Keywords

  • 16S rRNA
  • AmpC
  • Carbapenemase
  • ESBL
  • MBL

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