Eliminating the roughness in cholesterol's β-face: does it matter?

Martin R. Krause, Minghui Wang, Laurel Mydock-McGrane, Douglas F. Covey, Emmanuel Tejada, Paulo F. Almeida, Steven L. Regen

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

One of the long-standing issues surrounding cholesterol (Chol) relates to its two-faced character. In particular, the consequences of its having a rough β-face and a smooth α-face on its structural influence in cell membranes has remained elusive. In this study, direct comparisons have been made between cholesterol and a "smoothened" analog, DChol (i.e., 18,19-dinorcholesterol) using model membranes and a combination of nearest-neighbor recognition, differential scanning calorimetry, fluorescence, and monolayer measurements. Taken together, these results indicate that subtle differences exist between the interaction of these two sterols with the different states of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Chol has a greater condensing power than DChol, but only slightly so, i.e., on the order of a few tens of calories per mole.

Original languageEnglish
Pages (from-to)12114-12118
Number of pages5
JournalLangmuir : the ACS journal of surfaces and colloids
Volume30
Issue number41
DOIs
StatePublished - Oct 21 2014

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