Electrophysiological evidence for a rapid membrane action of the gonadal steroid, 17β-estradiol, on CA1 pyramidal neurons of the rat hippocampus

Micheal Wong; Robert L. Moss

doi:10.1016/0006-8993(91)91057-8

Electrophysiological evidence for a rapid membrane action of the gonadal steroid, 17β-estradiol, on CA1 pyramidal neurons of the rat hippocampus

Micheal Wong, Robert L. Moss

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Abstract

The rapid electrophysiological effects of 17β-estradiol on CA1 pyramidal neurons (n = 86) were investigated utilizing intracellular recording from the rat hippocampal slice preparation. Bath application of 17β-estradiol, but not 17α-estradiol, caused a reversible depolarization and increased input resistance with a latency of less than 1 min in 19.8% of CA1 neurons tested. There was no significant difference in the percentage of estradiol-responsive cells between male and female rats. Estradiol-responsive cells were identified from prepubertal female rats, as well as females in all stages of the estrous cycle. 17β-estradiol had no effect on the slow afterhyperpolarization or accommodative properties of CA1 neurons. In 2 out of 4 cells tested, the specific antiestrogen, tamoxifen, blocked the excitatory response to 17β-estradiol.

Original language	English
Pages (from-to)	148-152
Number of pages	5
Journal	Brain Research
Volume	543
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(91)91057-8
State	Published - Mar 8 1991

Keywords

CA1 pyramidal neuron
Estradiol
Hippocampal slice
Intracellular recording
Tamoxifen

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10.1016/0006-8993(91)91057-8

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title = "Electrophysiological evidence for a rapid membrane action of the gonadal steroid, 17β-estradiol, on CA1 pyramidal neurons of the rat hippocampus",

abstract = "The rapid electrophysiological effects of 17β-estradiol on CA1 pyramidal neurons (n = 86) were investigated utilizing intracellular recording from the rat hippocampal slice preparation. Bath application of 17β-estradiol, but not 17α-estradiol, caused a reversible depolarization and increased input resistance with a latency of less than 1 min in 19.8% of CA1 neurons tested. There was no significant difference in the percentage of estradiol-responsive cells between male and female rats. Estradiol-responsive cells were identified from prepubertal female rats, as well as females in all stages of the estrous cycle. 17β-estradiol had no effect on the slow afterhyperpolarization or accommodative properties of CA1 neurons. In 2 out of 4 cells tested, the specific antiestrogen, tamoxifen, blocked the excitatory response to 17β-estradiol.",

keywords = "CA1 pyramidal neuron, Estradiol, Hippocampal slice, Intracellular recording, Tamoxifen",

author = "Micheal Wong and Moss, {Robert L.}",

note = "Funding Information: We thank Angela Sherrin for technical assistance, Lisa Todd for assistance in preparing the manuscript, and Carol Dudley for comments on the manuscript. This work was supported by NIH Grants MH44591 and HD09988 Project V awarded to R.L.M.M.W. is a Medical Scientist Trainee supported by NIH Grant GM08014 and a gift from the Perot Family Foundation.",

year = "1991",

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doi = "10.1016/0006-8993(91)91057-8",

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AU - Moss, Robert L.

N1 - Funding Information: We thank Angela Sherrin for technical assistance, Lisa Todd for assistance in preparing the manuscript, and Carol Dudley for comments on the manuscript. This work was supported by NIH Grants MH44591 and HD09988 Project V awarded to R.L.M.M.W. is a Medical Scientist Trainee supported by NIH Grant GM08014 and a gift from the Perot Family Foundation.

PY - 1991/3/8

Y1 - 1991/3/8

N2 - The rapid electrophysiological effects of 17β-estradiol on CA1 pyramidal neurons (n = 86) were investigated utilizing intracellular recording from the rat hippocampal slice preparation. Bath application of 17β-estradiol, but not 17α-estradiol, caused a reversible depolarization and increased input resistance with a latency of less than 1 min in 19.8% of CA1 neurons tested. There was no significant difference in the percentage of estradiol-responsive cells between male and female rats. Estradiol-responsive cells were identified from prepubertal female rats, as well as females in all stages of the estrous cycle. 17β-estradiol had no effect on the slow afterhyperpolarization or accommodative properties of CA1 neurons. In 2 out of 4 cells tested, the specific antiestrogen, tamoxifen, blocked the excitatory response to 17β-estradiol.

AB - The rapid electrophysiological effects of 17β-estradiol on CA1 pyramidal neurons (n = 86) were investigated utilizing intracellular recording from the rat hippocampal slice preparation. Bath application of 17β-estradiol, but not 17α-estradiol, caused a reversible depolarization and increased input resistance with a latency of less than 1 min in 19.8% of CA1 neurons tested. There was no significant difference in the percentage of estradiol-responsive cells between male and female rats. Estradiol-responsive cells were identified from prepubertal female rats, as well as females in all stages of the estrous cycle. 17β-estradiol had no effect on the slow afterhyperpolarization or accommodative properties of CA1 neurons. In 2 out of 4 cells tested, the specific antiestrogen, tamoxifen, blocked the excitatory response to 17β-estradiol.

KW - CA1 pyramidal neuron

KW - Estradiol

KW - Hippocampal slice

KW - Intracellular recording

KW - Tamoxifen

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Electrophysiological evidence for a rapid membrane action of the gonadal steroid, 17β-estradiol, on CA1 pyramidal neurons of the rat hippocampus

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Keywords

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