Electrophysiological effects of amphiphiles on canine Purkinje fibers. Implications for dysrhythmia secondary to ischemia

P. B. Corr, D. W. Snyder, M. E. Cain, W. A. Crafford, R. W. Gross, B. E. Sobel

Research output: Contribution to journalArticlepeer-review

117 Scopus citations


Lysophosphoglycerides and long-chain acyl carnitines accumulate in ischemic myocardium soon after occlusion. Since both are amphiphiles and have some structural similarities, we studied their electrophysiological effects on transmembrane action potentials (AP) of isolated canine Purkinje fibers. Lysophosphatidyl choline (LPC) in the absence of albumin induced concentration-dependent (75-300 μM) and reversible decreases in maximum diastolic potential (MDP), AP amplitude, maximum upstroke velocity of phase 0 (V̇max) and action potential duration (APD). These responses were identical to those elicited by LPC in the presence of albumin at 10-fold higher concentrations (750-3000 μM). Palmitoyl carnitine induced similar concentration-dependent (75-300 μM) decreases in MDP, V̇max AP amplitude, and APD. In addition, at concentrations >100 μM, both compounds induced additional alterations characteristic of ischemia tissue in vivo: triangularization of the AP configuration, increased threshold for extracellular stimulation, conduction delay, non-uniform phase 0 depolarization and electrical alternans. Neither FFA, glycophosphoryl choline, nor carnitine, all possible catabolites of LPC or acyl carnitine, induced any significant electrophysiological alterations analogous to those caused by LPC. The derangements induced by LPC and palmitoyl carnitine were additive. Acidosis comparable to that seen during early ischemia in vivo (pH = 6.7) led to a 2- to 3-fold leftward shift in the concentration-response curve for LPC and palmitoyl carnitine. Acidosis alone without the amphiphile during the 10-minute superfusion period failed to alter MDP or AP amplitude, although V̇max was reduced (517 to 429 V/sec) and APD was prolonged rather than shortened. Thus, both lysophosphoglycerides and long-chain acyl carnitines increase in ischemic tissue and induce profound electrophysiological derangements closely analogous to those seen in ischemic myocardium in vivo, implicating both metabolites as potential progenitors of dysrhythmias during ischemia.

Original languageEnglish
Pages (from-to)354-363
Number of pages10
JournalUnknown Journal
Issue number2
StatePublished - 1981


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