TY - JOUR
T1 - Electron microscopy of living cells during in situ fluorescence microscopy
AU - Liv, Nalan
AU - Van Oosten Slingeland, Daan S.B.
AU - Baudoin, Jean Pierre
AU - Kruit, Pieter
AU - Piston, David W.
AU - Hoogenboom, Jacob P.
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2016/1/26
Y1 - 2016/1/26
N2 - We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.
AB - We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.
KW - Epidermal growth factor
KW - Fluorescence microscopy
KW - Live cell imaging
KW - Quantum dots
KW - Scanning electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=84989833630&partnerID=8YFLogxK
U2 - 10.1021/acsnano.5b03970
DO - 10.1021/acsnano.5b03970
M3 - Article
C2 - 26580231
AN - SCOPUS:84989833630
SN - 1936-0851
VL - 10
SP - 265
EP - 273
JO - ACS nano
JF - ACS nano
IS - 1
ER -