Electron microscopy of living cells during in situ fluorescence microscopy

Nalan Liv, Daan S.B. Van Oosten Slingeland, Jean Pierre Baudoin, Pieter Kruit, David W. Piston, Jacob P. Hoogenboom

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalACS nano
Issue number1
StatePublished - Jan 26 2016


  • Epidermal growth factor
  • Fluorescence microscopy
  • Live cell imaging
  • Quantum dots
  • Scanning electron microscopy


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