Elastase secretion by peritoneal exudative and alveolar macrophages*

Richard White, Hsiu San Lin, Charles Kuhn

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Mouse alveolar macrophages (AM) cultured in the absence of serum secrete an elastolytic enzyme. The elastase from AM resembles the previously described elastase from peritoneal macrophages (PM) in pH optimum and inhibition profile. The macrophage enzymes do not appear to be stored, and with periodic changes in the culture medium, accumulate extracellularly for up to 10 days. Resident PM produce barely detectable levels of extracellular elastase unless given a phagocytic load. Thioglycollate-stimulated peritoneal exudative macrophages (PEM), however, secrete easily detectable levels of elastase, which can be further increased with a phagocytic load. Without any additional stimulation, AM secrete an elastolytic activity comparable to that of the PEM receiving a phagocytic load, but unlike PM they do not increase elastase secretion after phagocytosis.

Original languageEnglish
Pages (from-to)802-808
Number of pages7
JournalJournal of Experimental Medicine
Volume146
Issue number3
DOIs
StatePublished - Sep 1 1977

Fingerprint Dive into the research topics of 'Elastase secretion by peritoneal exudative and alveolar macrophages*'. Together they form a unique fingerprint.

  • Cite this