Abstract

The production of PGE2 6-keto PGF, and TxB2 under basal conditions and after exposure to angiotensin II was examined in vitro in isolated glomeruli from sham-operated control rats and rats with unilateral ureteral obstruction of 24 hour duration, that were or were not pretreated with an inhibitor of the angiotensin I converting enzyme (ACE). Basal prostanoid production was greater in glomeruli from the obstructed kidney (OK) than in glomeruli from the contralateral kidney (CLK) of rats with obstruction or glomeruli from the kidneys of sham-operated rats. Glomeruli obtained from the CLK of rats with unilateral obstruction also produced more PGE2 and 6-keto PGF, than glomeruli obtained from kidneys of sham-operated rats. Administration of an ACE inhibitor to rats with unilateral obstruction in vivo returned basal prostanoid production in vitro to levels seen in glomeruli of sham-operated rats. The increase in prostanoid production in response to angiotensin II added in vitro was less in glomeruli from rats with unilateral obstruction than in glomeruli from sham-operated rats. However, the response was restored to that seen in glomeruli of sham-operated rats after blockade of angiotensin II synthesis in vivo in rats with unilateral obstruction. Blockade of angiotensin II synthesis in sham-operated rats did not affect prostanoid synthesis by their glomeruli. The results indicate that endogenous angiotensin II has an important role in the increased synthesis of prostanoids found not only in glomeruli of the OK but also of the CLK of rats with unilateral obstruction and that the in vitro prostanoid production in response to angiotensin II can be restored to that seen in sham-operated rats when the synthesis of angiotensin II is inhibited in vivo.

Original languageEnglish
Pages (from-to)1528-1535
Number of pages8
JournalKidney International
Volume37
Issue number6
DOIs
StatePublished - Jun 1990

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