Efficient Transduction of Zebrafish Melanoma Cell Lines and Embryos Using Lentiviral Vectors

Maurizio Fazio, Serine Avagyan, Ellen Van Rooijen, William Mannherz, Charles K. Kaufman, Riadh Lobbardi, David M. Langenau, Leonard I. Zon

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The establishment of in vitro cultures of zebrafish cancer cells has expanded the potential of zebrafish as a disease model. However, the lack of effective methods for gene delivery and genetic manipulation has limited the experimental applications of these cultures. To overcome this barrier, we tested and optimized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviral and retroviral vector transduction protocols. We show that lentivirus successfully and efficiently transduced zebrafish melanoma cell lines in vitro, allowing antibiotic selection, fluorescence-based sorting, and in vivo allotransplantation. In addition, injection of concentrated lentiviral particles into embryos and tumors established the feasibility of in vivo gene delivery.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalZebrafish
Volume14
Issue number4
DOIs
StatePublished - Aug 2017

Keywords

  • Viral vectors
  • gene delivery
  • lentiviral
  • lentivirus
  • retrovirus
  • zebrafish cell lines

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