Efficient mammalian cell expression and single-step purification of extracellular glycoproteins for crystallization

Keyphrases

• Glycoprotein 100%
• Crystallization 100%
• One-step Purification 100%
• Mammalian Cell Expression 100%
• Mammalian Cells 66%
• Transfection Reagent 66%
• Mammalian Proteins 66%
• Virus 33%
• Protein Expression 33%
• Transient Transfection 33%
• X-ray Crystallography 33%
• Cytotoxicity 33%
• Glucose Level 33%
• Secreted Proteins 33%
• Chemical Modification 33%
• Glycans 33%
• Biophysical Studies 33%
• Transfection Efficiency 33%
• Final Yield 33%
• Protein Yield 33%
• Efficient Protocol 33%
• Nickel Affinity Chromatography 33%

Biochemistry, Genetics and Molecular Biology

• Transfection 100%
• Glycoprotein 100%
• Copurification 100%
• Maturation 33%
• Protein Expression 33%
• Transient Transfection 33%
• Cytotoxicity 33%
• Glucose Level 33%
• X-Ray Crystallography 33%
• Virus Expression 33%
• Polysaccharide 33%
• Affinity Chromatography 33%