Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Keyphrases

• Caenorhabditis Elegans 100%
• Deletion Strain 100%
• Restriction Endonuclease 100%
• PCR Method 66%
• PCR Products 66%
• Efficiency Increase 66%
• Gene Knockout 33%
• Gene Function 33%
• Targeted Deletion 33%
• Reverse Genetics 33%
• Genetic Approach 33%
• Restriction Enzyme 33%
• Deletion mutant 33%
• Multiplexing 33%
• Diploid Genome 33%
• DNA Samples 33%
• Primer Pairs 33%
• Detection Sensitivity 33%
• Small Deletion 33%
• Novel Modification 33%
• PCR Detection 33%
• Nonspecific Amplification 33%
• Method Efficiency 33%
• Small Screen 33%

Biochemistry, Genetics and Molecular Biology

• Caenorhabditis Elegans 100%
• Restriction Modification System 100%
• Wild Type 33%
• Gene Function 33%
• Gene Knockout 33%
• Deletion Mutant 33%
• Genetic Approach 33%
• Restriction Enzyme 33%
• reverse genetics 33%