Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans

Indrani Bose; Tamara L. Doering

doi:10.1016/j.mimet.2011.04.014

Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans

Indrani Bose, Tamara L. Doering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

An improved method has been developed for RNA interference in Cryptococcus neoformans, using opposing promoters to facilitate cloning and RNA interference targeting URA5 to allow selection of cells in which silencing is most effective. These advances significantly reduce the variability of silencing and the effort required for interference plasmid construction.

Original language	English
Pages (from-to)	156-159
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2011.04.014
State	Published - Aug 2011

Keywords

Cryptococcus neoformans
Pathogenic fungus
RNA interference

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10.1016/j.mimet.2011.04.014

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title = "Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans",

abstract = "An improved method has been developed for RNA interference in Cryptococcus neoformans, using opposing promoters to facilitate cloning and RNA interference targeting URA5 to allow selection of cells in which silencing is most effective. These advances significantly reduce the variability of silencing and the effort required for interference plasmid construction.",

keywords = "Cryptococcus neoformans, Pathogenic fungus, RNA interference",

author = "Indrani Bose and Doering, {Tamara L.}",

note = "Funding Information: This work was supported by National Institutes of Health grant GM071007 and an Award from the Edward J. Mallinckrodt, Jr. Foundation . The authors are grateful to Morgann C. Reilly for plasmid sequencing, Michael L. Skowyra for comments on the manuscript and sequence submission, and Doering lab members for helpful suggestions.",

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AU - Doering, Tamara L.

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KW - Pathogenic fungus

KW - RNA interference

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ER -

Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans

Abstract

Keywords

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