Efficient generation of targeted and controlled mutational events in porcine cells using nuclease-directed homologous recombination

Keyphrases

• Efficient Generation 100%
• Homologous Recombination 100%
• Nuclease 100%
• Mutational Event 100%
• Porcine Cells 100%
• Cas9 Protein 57%
• Single-stranded DNA (ssDNA) 57%
• Non-integrating 42%
• Porcine Genome 42%
• Genome Editing 28%
• Locus-specific 28%
• Single Guide RNA (sgRNA) 28%
• Mutation Rate 28%
• DNA Oligonucleotides 28%
• Palindromic Repeat 28%
• Cell-based 14%
• Genetic Modification 14%
• MiRNA Target 14%
• Co-transfection 14%
• Flow Cytometry 14%
• Green Fluorescent Protein 14%
• Homology-directed Repair 14%
• Plasmid 14%
• Site-directed mutagenesis 14%
• Flow Cytometer 14%
• Restriction Enzyme 14%
• Selection Strategy 14%
• Insertion or Deletion 14%
• Sanger Sequencing 14%
• Human Disease Models 14%
• Protein System 14%
• Transgene Expression 14%
• Restriction Sites 14%
• Start Codon 14%
• Deletion mutation 14%
• Biallelic 14%
• Immunodeficient 14%
• Transgene Delivery 14%
• Insertion mutation 14%
• Selection Marker 14%
• Editing Tools 14%
• Fluorescence Proteins 14%
• Clinical Xenotransplantation 14%
• GGTA1 14%
• α1,3-galactosyltransferase 14%

Biochemistry, Genetics and Molecular Biology

• Pig 100%
• Homologous Recombination 100%
• Nuclease 100%
• Transgene 42%
• DNA Strand 42%
• Cas9 Protein 42%
• Oligonucleotide 28%
• Mutation Rate 28%
• Guide RNA 28%
• Cas9 14%
• CRISPR/Cas9 14%
• Transfection 14%
• Green Fluorescent Protein 14%
• Mutagenesis 14%
• DNA Template 14%
• Plasmid 14%
• Genome Editing 14%
• DNA Modification 14%
• Restriction Site 14%
• Start Codon 14%
• Galactosyltransferase 14%
• Genomics 14%
• Flow Cytometry 14%
• Restriction Enzyme 14%