Efficient gene transfer to human endothelial cells using DNA complexed to adenovirus particles

Cora Jean S. Edgell, David T. Curiel, Ping Chuan Hu, Henry S. Marr

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Human endothelial cells have been found to be relatively refractory to various methods of DNA transfection currently in common use. By using a transfection method involving DNA complexed with replication-deficient adenovirus particles, we have shown that 20% of a population of cultured endothelial cells can be transfected and high levels of transient expression achieved. Both early-passage human umbilical vein endothelial cells and the continuous differentiated line of human endothelium-derived EA.hy926 cells are responsive to this method of transfection. Efficient DNA transfection of endothelial cells is important for studies of endothelium-specific promoters and is a potentially useful route for transgenic therapy.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalBioTechniques
Volume25
Issue number2
DOIs
StatePublished - 1998

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