Effects of Vasopressin on Blood‐Brain Transfer of Methionine in Dogs

Peter Brust, Elias K. Shaya, Keith J. Jeffries, Robert F. Dannals, Hayden T. Ravert, Alan A. Wilson, Peter S. Conti, Henry N. Wagner, Albert Gjedde, Armin Ermisch, Dean F. Wong

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Abstract: We used a simplified probe detection system for positron‐emitting radionuclides in order to measure blood‐brain barrier transport of amino acids in anesthetized dogs. Plasma and brain time‐activity curves were recorded after intravenous bolus injection of L‐[11C]methionine before and after administration of 1 μg of vasopressin. Three‐compartment models with three or four transfer coefficients were used to derive the kinetics of L‐[11C]methionine uptake in brain. The blood‐brain clearance of the tracer (K1) was 0.075 ml ml‐1 min‐1 before and 0.041 ml ml‐1 mir‐1 after injection of vasopressin. The partition volume and the initial distribution (plasma) volume of methionine were unchanged and within the expected limits. The net accumulation rate of methionine (K), estimated by both the four‐parameter (kinetic) and three‐parameter (graphic) approaches, decreased after vasopressin injection in all six studies.

Original languageEnglish
Pages (from-to)1421-1429
Number of pages9
JournalJournal of Neurochemistry
Volume59
Issue number4
DOIs
StatePublished - Oct 1992
Externally publishedYes

Keywords

  • Amino acid
  • Blood‐brain barrier
  • Dog
  • Endothelial cell
  • In vivo method
  • Methionine
  • Permeability‐surface area product
  • Positron emission tomography
  • Transport

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