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Effects of temperature and ATP on the kinetic mechanism and kinetic step-size for E. coli RecBCD helicase-catalyzed DNA unwinding
Aaron L. Lucius
,
Timothy M. Lohman
Department of Biochemistry & Molecular Biophysics
Roy and Diana Vagelos Division of Biology & Biomedical Sciences (DBBS)
Institute of Clinical and Translational Sciences (ICTS)
Siteman Cancer Center
DBBS - Biochemistry, Biophysics, and Structural Biology
Research output
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Contribution to journal
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Article
›
peer-review
44
Scopus citations
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Dive into the research topics of 'Effects of temperature and ATP on the kinetic mechanism and kinetic step-size for E. coli RecBCD helicase-catalyzed DNA unwinding'. Together they form a unique fingerprint.
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Keyphrases
Escherichia Coli
100%
Helicase
100%
DNA Unwinding
100%
RecBCD
100%
Kinetic Mechanism
100%
Temperature Effect
100%
Duplex DNA
50%
Single Turnover
33%
Rate Limiting
16%
Glycerol
16%
Sodium Chloride
16%
DNA Substrates
16%
Magnesium Chloride
16%
Single-molecule Fluorescence Resonance Energy Transfer (smFRET)
16%
Fluorescently Labeled
16%
K(I)
16%
Rate-limiting Step
16%
ATP Binding
16%
Increased Temperature
16%
Maximum Rate
16%
Stopped-flow Technique
16%
Labeled DNA
16%
Elementary Kinetics
16%
Constant Change
16%
Biochemistry, Genetics and Molecular Biology
Adenosine Triphosphate
100%
Escherichia coli
100%
DNA Denaturation
100%
Helicase
100%
RecBCD
100%
Fluorescence Resonance Energy Transfer
16%
Glycerol
16%