TY - JOUR
T1 - Effects of substrate stiffness on adipogenic and osteogenic differentiation of human mesenchymal stem cells
AU - Zhao, Wen
AU - Li, Xiaowei
AU - Liu, Xiaoyan
AU - Zhang, Ning
AU - Wen, Xuejun
N1 - Funding Information:
This work was made possible by the National Science Foundation CAREER ( 1055922 ), Wallace H. Coulter Foundation , NIH/NINDS USA ( R01 NS050243 ), and American Heart Association ( 10PRE4280017 ). We thank Jane Jordan from the Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina (MUSC) for her generous help with RT-PCR.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Substrate mechanical properties, in addition to biochemical signals, have been shown to modulate cell phenotype. In this study, we inspected the effects of substrate stiffness on human mesenchymal stem cells (hMSCs) derived from adult human bone marrow differentiation into adipogenic and osteogenic cells. A chemically modified extracellular matrix derived and highly biocompatible hydrogel, based on thiol functionalized hyaluronic acid (HA-SH) and thiol functionalized recombinant human gelatin (Gtn-SH), which can be crosslinked by poly (ethylene glycol) tetra-acrylate (PEGTA), was used as a model system. The stiffness of the hydrogel was controlled by adjusting the crosslinking density. Human bone marrow MSCs were cultured on the hydrogels with different stiffness under adipogenic and osteogenic conditions. Oil Red O staining and F-actin staining were applied to assess the change of cell morphologies under adipogenic and osteogenic differentiation, respectively. Gene expression of cells was determined with reverse transcription polymerase chain reaction (RT-PCR) as a function of hydrogel stiffness. Results support the hypothesis that adipogenic and osteogenic differentiation of hMSCs are inclined to occur on substrate with stiffness similar to their in vivo microenvironments.
AB - Substrate mechanical properties, in addition to biochemical signals, have been shown to modulate cell phenotype. In this study, we inspected the effects of substrate stiffness on human mesenchymal stem cells (hMSCs) derived from adult human bone marrow differentiation into adipogenic and osteogenic cells. A chemically modified extracellular matrix derived and highly biocompatible hydrogel, based on thiol functionalized hyaluronic acid (HA-SH) and thiol functionalized recombinant human gelatin (Gtn-SH), which can be crosslinked by poly (ethylene glycol) tetra-acrylate (PEGTA), was used as a model system. The stiffness of the hydrogel was controlled by adjusting the crosslinking density. Human bone marrow MSCs were cultured on the hydrogels with different stiffness under adipogenic and osteogenic conditions. Oil Red O staining and F-actin staining were applied to assess the change of cell morphologies under adipogenic and osteogenic differentiation, respectively. Gene expression of cells was determined with reverse transcription polymerase chain reaction (RT-PCR) as a function of hydrogel stiffness. Results support the hypothesis that adipogenic and osteogenic differentiation of hMSCs are inclined to occur on substrate with stiffness similar to their in vivo microenvironments.
KW - Differentiation
KW - Hydrogel
KW - Mechanical property
KW - Mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=84899654691&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2014.03.048
DO - 10.1016/j.msec.2014.03.048
M3 - Article
C2 - 24857499
AN - SCOPUS:84899654691
SN - 0928-4931
VL - 40
SP - 316
EP - 323
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -