Effects of progestins and relaxin on glycodelin gene expression in human endometrial cells

Robert N. Taylor, Jean Louis Vigne, Peilin Zhang, Phuong Hoang, Dan I. Lebovic, Michael D. Mueller

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


OBJECTIVE: Glycodelin is an endometrial protein proposed to play an important role in embryonic implantation. We examined the effects of progestins and relaxin on glycodelin transcription, synthesis, and secretion. STUDY DESIGN: Northern blotting, metabolic labeling, and fluorography were used to assess glycodelin messenger ribonucleic acid and protein synthesis in endometrial tissue and cells. Luciferase reporter constructs transfected into endometrial adenocarcinoma cells ([shikawa cells) were used to determine whether progestins or relaxin could activate the glycodelin gene promoter. RESULTS: Progestins but not relaxin stimulated glycodelin secretion in primary epithelial cell cultures. A 452-base pair fragment of the glycodelin gene promoter was activated 4.3 ± 0.7 times normal by 10nmol/L promegestone; however, addition of relaxin to the same construct repressed progestin- stimulated promoter activation by >30%. CONCLUSION: Glycodelin transcription, synthesis, and secretion by endometrial epithelial cells were stimulated by progestins. However, relaxin failed to stimulate production of this immunomodulatory protein and, in fact, repressed progestin-stimulated activation of the glycodelin gene promoter.

Original languageEnglish
Pages (from-to)841-849
Number of pages9
JournalAmerican journal of obstetrics and gynecology
Issue number4
StatePublished - 2000


  • Glycodelin
  • Implantation
  • Placental protein 14
  • Uterus


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