TY - JOUR
T1 - Effects of extracellular matrix upon elastogenesis
AU - Mecham, Robert P.
N1 - Funding Information:
This work was supported by grant HL 16118 from the National Institutes of Health. Robert Mecham' is an Established Investigator of the American Heart Association.
PY - 1981
Y1 - 1981
N2 - Bovine fetal ligamentum nuchae fibroblasts were cultured on tissue culture plastic or directly on killed fetal ligament tissue. Soluble elastin production and formation of insoluble elastin where monitored by radioimmunoassay and incorporation of [14C] lysine into desmosine, respectively. Cells grown on tissue culture plastic synthesized elastin precursors but were unable to form insoluble elastin whereas cells grown on dead ligament not only formed insoluble elastin, but showed an increase in levels of immunoreactive soluble elastin in culture medium. Stimulation of elastin synthesis by a soluble factor originating in ligament tissue is unlikely since medium conditioned by viable or nonviable ligament tissue had no effect on ligament cells cultured on plastic. Thus, direct cell-matrix contact appears to be important in mediating elastin biosynthesis.
AB - Bovine fetal ligamentum nuchae fibroblasts were cultured on tissue culture plastic or directly on killed fetal ligament tissue. Soluble elastin production and formation of insoluble elastin where monitored by radioimmunoassay and incorporation of [14C] lysine into desmosine, respectively. Cells grown on tissue culture plastic synthesized elastin precursors but were unable to form insoluble elastin whereas cells grown on dead ligament not only formed insoluble elastin, but showed an increase in levels of immunoreactive soluble elastin in culture medium. Stimulation of elastin synthesis by a soluble factor originating in ligament tissue is unlikely since medium conditioned by viable or nonviable ligament tissue had no effect on ligament cells cultured on plastic. Thus, direct cell-matrix contact appears to be important in mediating elastin biosynthesis.
UR - http://www.scopus.com/inward/record.url?scp=0019410094&partnerID=8YFLogxK
U2 - 10.3109/03008208109152382
DO - 10.3109/03008208109152382
M3 - Article
C2 - 6455252
AN - SCOPUS:0019410094
VL - 8
SP - 241
EP - 244
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 3-4
ER -