TY - JOUR
T1 - Effects of ethanol and acetaldehyde on the biosynthesis of testosterone in the rodent testes
AU - Cicero, Theodore J.
AU - Bell, Roy D.
N1 - Funding Information:
research was supported in part by USPHS grants AA-03242 and AA-03539. is a recipient of Research Scientist Development Award AA-70180.
PY - 1980/6/16
Y1 - 1980/6/16
N2 - The effects of ethanol and acetaldehyde on testicular steroidogenesis were examined in enzymatically dispersed cells of the rodent testes. Both drugs significantly inhibited gonadotropin-stimulated steroidogenesis, but acetaldehyde was considerably more potent (>1000 times) than ethanol. To determine the step in testosterone's biosynthetic pathway which was inhibited by the two drugs, cells were incubated in the presence of [3H]pregnenolone and [3H]progesterone, and the amount of label incorporated into testosterone and its precursors was determined. Ethanol and acetaldehyde inhibited only the conversion of androstenedione to testosterone; none of the other precursors of testosterone was affected.
AB - The effects of ethanol and acetaldehyde on testicular steroidogenesis were examined in enzymatically dispersed cells of the rodent testes. Both drugs significantly inhibited gonadotropin-stimulated steroidogenesis, but acetaldehyde was considerably more potent (>1000 times) than ethanol. To determine the step in testosterone's biosynthetic pathway which was inhibited by the two drugs, cells were incubated in the presence of [3H]pregnenolone and [3H]progesterone, and the amount of label incorporated into testosterone and its precursors was determined. Ethanol and acetaldehyde inhibited only the conversion of androstenedione to testosterone; none of the other precursors of testosterone was affected.
UR - http://www.scopus.com/inward/record.url?scp=0019309437&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(80)91307-8
DO - 10.1016/0006-291X(80)91307-8
M3 - Article
C2 - 6772181
AN - SCOPUS:0019309437
SN - 0006-291X
VL - 94
SP - 814
EP - 819
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -