TY - JOUR
T1 - Effects of early cold stress on gene expression in Chlamydomonas reinhardtii
AU - Li, Lun
AU - Peng, Hai
AU - Tan, Shenglong
AU - Zhou, Junfei
AU - Fang, Zhiwei
AU - Hu, Zhangfeng
AU - Gao, Lifen
AU - Li, Tiantian
AU - Zhang, Weixiong
AU - Chen, Lihong
N1 - Funding Information:
The work was supported by Department of Education of Hubei Province (B2016295). All the RNA-seq data were submitted to the National Center for Biotechnology Information under SRP number SRP125522. LHC performed the research, designed the experiments and wrote the manuscript. LL, PH, SLT and ZWF carried out computational analysis. LLL performed the Real-time validation. JFZ, LFG and TTL constructed sequencing libraries and preformed sequencing profiling. ZFH provided the algae and performed the cold treatment. HP and WXZ revised the manuscript. All authors read and approved the final manuscript. None declared.
Funding Information:
The work was supported by Department of Education of Hubei Province ( B2016295 ).
Publisher Copyright:
© 2019
PY - 2020/3
Y1 - 2020/3
N2 - Cold stress imposes a great impact on the growth of nearly all photosynthetic organisms, including Chlamydomonas reinhardtii (C. reinhardtii). Despite prior studies on the mechanism of stress acclimation in plants, little has been done on the early events of cold sensing in C. reinhardtii. Here, we used C. reinhardtii as a model to study early events of cold signal transduction. By analyzing transcriptomic changes of C. reinhardtii exposed to cold, we found that 3471 genes were differentially expressed after 1 h of cold exposure. These genes were associated with a wide range of biological events and processes such as protein synthesis, cell cycle and protein kinase-based phosphorylation. Besides, the promoter of one gene (named as crAP2) which belongs to AP2/EREBP family and was significantly induced by cold was cloned, and functional analysis was conducted using GUS activity analysis through Agrobacterium-mediated transient assay in tobacco leaves.
AB - Cold stress imposes a great impact on the growth of nearly all photosynthetic organisms, including Chlamydomonas reinhardtii (C. reinhardtii). Despite prior studies on the mechanism of stress acclimation in plants, little has been done on the early events of cold sensing in C. reinhardtii. Here, we used C. reinhardtii as a model to study early events of cold signal transduction. By analyzing transcriptomic changes of C. reinhardtii exposed to cold, we found that 3471 genes were differentially expressed after 1 h of cold exposure. These genes were associated with a wide range of biological events and processes such as protein synthesis, cell cycle and protein kinase-based phosphorylation. Besides, the promoter of one gene (named as crAP2) which belongs to AP2/EREBP family and was significantly induced by cold was cloned, and functional analysis was conducted using GUS activity analysis through Agrobacterium-mediated transient assay in tobacco leaves.
KW - Chlamydomonas reinhardtii
KW - Cold stress
KW - Early signaling events
KW - Expression pattern
KW - Promoter
UR - https://www.scopus.com/pages/publications/85069599838
U2 - 10.1016/j.ygeno.2019.06.027
DO - 10.1016/j.ygeno.2019.06.027
M3 - Article
C2 - 31251979
AN - SCOPUS:85069599838
SN - 0888-7543
VL - 112
SP - 1128
EP - 1138
JO - Genomics
JF - Genomics
IS - 2
ER -