TY - JOUR
T1 - Effects of dietary β-glucan on the growth, immune responses and resistance of sea cucumber, Apostichopus japonicus against Vibrio splendidus infection
AU - Zhao, Yancui
AU - Ma, Hongming
AU - Zhang, Wenbing
AU - Ai, Qinghui
AU - Mai, Kangsen
AU - Xu, Wei
AU - Wang, Xiaojie
AU - Liufu, Zhiguo
N1 - Funding Information:
The work was supported by the National High Technology Research and Development Program (“863”Program) of China (Grant No.: 2006AA100313 ).
PY - 2011/5/21
Y1 - 2011/5/21
N2 - An eight-week feeding trial was conducted to determine the effects of dietary supplementation of β-glucan on the growth, immunity and resistance of sea cucumber against Vibrio splendidus infection. A basal diet was formulated to contain 20.6% crude protein and 4.8% crude lipid. Two levels (1.25 and 2.50. g/kg) of β-glucan were added to the basal diet to replace wheat. After the feeding trial, a V. splendidus injection challenge was executed to test the effects of β-glucan on disease resistance. Enhanced growth was observed in sea cucumber fed diet supplemented with 1.25. g glucan/kg, but not in sea cucumber fed diet supplemented with 2.50. g glucan/kg. The coelomocyte phagocytosis activity of sea cucumber fed β-glucan-supplemented diets was significantly (P<. 0.05) higher than those of sea cucumbers fed the basal diet. In addition, phenoloxidase activity of coelomocytes was significantly (P<. 0.05) enhanced by dietary supplementation of β-glucan. Sea cucumbers fed 1.25. g/kg glucan had a significant (P<. 0.05) increase in respiratory burst of coelomocytes, compared to sea cucumber fed diet containing 2.50. g β-glucan/kg; however, coelomocyte respiratory burst of sea cucumber fed 2.50. g β-glucan/kg diet was not significantly different from those of sea cucumber fed the basal diet. The challenge test showed that dietary supplementation of β-glucan at inclusion level of 1.25. g/kg conferred significant protection to sea cucumber against V. splendidus infection. However, protective effect of β-glucan supplementation at 2.50. g/kg was marginal. It is concluded that dietary β-glucan has potential for use in diet formulations of sea cucumber to limit the adverse effects of V. splendidus, however, dosage should be an important consideration in administration.
AB - An eight-week feeding trial was conducted to determine the effects of dietary supplementation of β-glucan on the growth, immunity and resistance of sea cucumber against Vibrio splendidus infection. A basal diet was formulated to contain 20.6% crude protein and 4.8% crude lipid. Two levels (1.25 and 2.50. g/kg) of β-glucan were added to the basal diet to replace wheat. After the feeding trial, a V. splendidus injection challenge was executed to test the effects of β-glucan on disease resistance. Enhanced growth was observed in sea cucumber fed diet supplemented with 1.25. g glucan/kg, but not in sea cucumber fed diet supplemented with 2.50. g glucan/kg. The coelomocyte phagocytosis activity of sea cucumber fed β-glucan-supplemented diets was significantly (P<. 0.05) higher than those of sea cucumbers fed the basal diet. In addition, phenoloxidase activity of coelomocytes was significantly (P<. 0.05) enhanced by dietary supplementation of β-glucan. Sea cucumbers fed 1.25. g/kg glucan had a significant (P<. 0.05) increase in respiratory burst of coelomocytes, compared to sea cucumber fed diet containing 2.50. g β-glucan/kg; however, coelomocyte respiratory burst of sea cucumber fed 2.50. g β-glucan/kg diet was not significantly different from those of sea cucumber fed the basal diet. The challenge test showed that dietary supplementation of β-glucan at inclusion level of 1.25. g/kg conferred significant protection to sea cucumber against V. splendidus infection. However, protective effect of β-glucan supplementation at 2.50. g/kg was marginal. It is concluded that dietary β-glucan has potential for use in diet formulations of sea cucumber to limit the adverse effects of V. splendidus, however, dosage should be an important consideration in administration.
KW - Apostichopus japonicus
KW - Disease challenge
KW - Growth
KW - Immunity response
KW - Vibrio splendidus
KW - β-glucan
UR - http://www.scopus.com/inward/record.url?scp=79955035029&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2011.02.032
DO - 10.1016/j.aquaculture.2011.02.032
M3 - Article
AN - SCOPUS:79955035029
SN - 0044-8486
VL - 315
SP - 269
EP - 274
JO - Aquaculture
JF - Aquaculture
IS - 3-4
ER -