Effects of barium and ion substitutions in artificial blood on endocochlear potential

Daniel C. Marcus; Masaaki Rokugo; Rüdiger Thalmann

doi:10.1016/0378-5955(85)90133-9

Effects of barium and ion substitutions in artificial blood on endocochlear potential

Daniel C. Marcus, Masaaki Rokugo, Rüdiger Thalmann

Department of Otolaryngology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Previously, a qualitative assessment was made (Marcus, D.C. (1984): Am. J. Physiol. 247, C240-C246) of the ion-selective properties of the cells bounding the cochlear duct by observing the effects of ion substitutions in the perilymph on the transepithelial potential difference (endocochlear potential; EP). Contributions by the marginal cells of the stria vascularis to the observed changes in the EP may have been masked, however, due to their 'isolation' from the perilymph by a continuous layer of basal cells. Since the ionic milieu of the basolateral membranes of the marginal cells is controlled more directly by the blood supply than by the perilymph, we report here on the effects of ion substitutions via vascular perfusion. Elevated K (substituted for Na or N-methyl-d-glucamine; NMDG) or Ba caused marked depression of the EP. Decreased Na or Cl (replaced by NMDG and gluconate, respectively) also depressed the EP. These changes in the EP were distinctly different from those observed previously by perilymphatic perfusion, and were interpreted in terms of a modified model of the striai marginal cells.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Hearing research
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/0378-5955(85)90133-9
State	Published - Jan 1985

Keywords

barium
cell model
endocochlear potential
guinea pig
ion substitution

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@article{18a5bf72fb0f44ceab23ef6dad73235e,

title = "Effects of barium and ion substitutions in artificial blood on endocochlear potential",

abstract = "Previously, a qualitative assessment was made (Marcus, D.C. (1984): Am. J. Physiol. 247, C240-C246) of the ion-selective properties of the cells bounding the cochlear duct by observing the effects of ion substitutions in the perilymph on the transepithelial potential difference (endocochlear potential; EP). Contributions by the marginal cells of the stria vascularis to the observed changes in the EP may have been masked, however, due to their 'isolation' from the perilymph by a continuous layer of basal cells. Since the ionic milieu of the basolateral membranes of the marginal cells is controlled more directly by the blood supply than by the perilymph, we report here on the effects of ion substitutions via vascular perfusion. Elevated K (substituted for Na or N-methyl-d-glucamine; NMDG) or Ba caused marked depression of the EP. Decreased Na or Cl (replaced by NMDG and gluconate, respectively) also depressed the EP. These changes in the EP were distinctly different from those observed previously by perilymphatic perfusion, and were interpreted in terms of a modified model of the striai marginal cells.",

keywords = "barium, cell model, endocochlear potential, guinea pig, ion substitution",

author = "Marcus, {Daniel C.} and Masaaki Rokugo and R{\"u}diger Thalmann",

note = "Funding Information: The authorst hankD r. E. Arakawaf or performing a few of the perfusione xperimentsT. his work was supportedb y National Instituteso f Health grantsN S 19490a nd NS 06575.",

year = "1985",

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doi = "10.1016/0378-5955(85)90133-9",

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AU - Rokugo, Masaaki

AU - Thalmann, Rüdiger

N1 - Funding Information: The authorst hankD r. E. Arakawaf or performing a few of the perfusione xperimentsT. his work was supportedb y National Instituteso f Health grantsN S 19490a nd NS 06575.

PY - 1985/1

Y1 - 1985/1

N2 - Previously, a qualitative assessment was made (Marcus, D.C. (1984): Am. J. Physiol. 247, C240-C246) of the ion-selective properties of the cells bounding the cochlear duct by observing the effects of ion substitutions in the perilymph on the transepithelial potential difference (endocochlear potential; EP). Contributions by the marginal cells of the stria vascularis to the observed changes in the EP may have been masked, however, due to their 'isolation' from the perilymph by a continuous layer of basal cells. Since the ionic milieu of the basolateral membranes of the marginal cells is controlled more directly by the blood supply than by the perilymph, we report here on the effects of ion substitutions via vascular perfusion. Elevated K (substituted for Na or N-methyl-d-glucamine; NMDG) or Ba caused marked depression of the EP. Decreased Na or Cl (replaced by NMDG and gluconate, respectively) also depressed the EP. These changes in the EP were distinctly different from those observed previously by perilymphatic perfusion, and were interpreted in terms of a modified model of the striai marginal cells.

AB - Previously, a qualitative assessment was made (Marcus, D.C. (1984): Am. J. Physiol. 247, C240-C246) of the ion-selective properties of the cells bounding the cochlear duct by observing the effects of ion substitutions in the perilymph on the transepithelial potential difference (endocochlear potential; EP). Contributions by the marginal cells of the stria vascularis to the observed changes in the EP may have been masked, however, due to their 'isolation' from the perilymph by a continuous layer of basal cells. Since the ionic milieu of the basolateral membranes of the marginal cells is controlled more directly by the blood supply than by the perilymph, we report here on the effects of ion substitutions via vascular perfusion. Elevated K (substituted for Na or N-methyl-d-glucamine; NMDG) or Ba caused marked depression of the EP. Decreased Na or Cl (replaced by NMDG and gluconate, respectively) also depressed the EP. These changes in the EP were distinctly different from those observed previously by perilymphatic perfusion, and were interpreted in terms of a modified model of the striai marginal cells.

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KW - cell model

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Effects of barium and ion substitutions in artificial blood on endocochlear potential

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Keywords

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