Effects of AMD3100 on transmigration and survival of acute myelogenous leukemia cells

Jane L. Liesveld; Jeremy Bechelli; Karen Rosell; Chaohui Lu; Gary Bridger; Gordon Phillips; Camille N. Abboud

doi:10.1016/j.leukres.2007.02.017

Effects of AMD3100 on transmigration and survival of acute myelogenous leukemia cells

Jane L. Liesveld, Jeremy Bechelli, Karen Rosell, Chaohui Lu, Gary Bridger, Gordon Phillips, Camille N. Abboud

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Acute myelogenous leukaemia (AML) blasts transmigrate in response to SDF-1α. AMD3100, a novel bicyclam molecule which inhibits stromal-derived factor (SDF)-1α/CXCR4 interactions, inhibited the transmigration of AML blasts and inhibited outgrowth of leukemia colony forming units. AMD3100 did not abrogate stroma-mediated protection from cytarabine-mediated apoptosis, except in the case of one promyelocytic leukemic sample tested, and it did not influence adhesion of blasts to endothelial monolayers. When AML blasts were pretreated with AMD3100, the positive effects of SDF-1α on NOD/SCID engraftment were diminished. This work confirms that AML is influenced by the SDF-1α/CXCR4 axis and demonstrates that disruption of this axis by the bicyclam AMD3100 can influence AML microenvironmental interactions.

Original language	English
Pages (from-to)	1553-1563
Number of pages	11
Journal	Leukemia Research
Volume	31
Issue number	11
DOIs	https://doi.org/10.1016/j.leukres.2007.02.017
State	Published - Nov 2007

Keywords

AMD3100
Acute leukemia
CXCR4/SDF-1α
Transmigration

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@article{1ac03b78d97c40379687320e144af294,

title = "Effects of AMD3100 on transmigration and survival of acute myelogenous leukemia cells",

abstract = "Acute myelogenous leukaemia (AML) blasts transmigrate in response to SDF-1α. AMD3100, a novel bicyclam molecule which inhibits stromal-derived factor (SDF)-1α/CXCR4 interactions, inhibited the transmigration of AML blasts and inhibited outgrowth of leukemia colony forming units. AMD3100 did not abrogate stroma-mediated protection from cytarabine-mediated apoptosis, except in the case of one promyelocytic leukemic sample tested, and it did not influence adhesion of blasts to endothelial monolayers. When AML blasts were pretreated with AMD3100, the positive effects of SDF-1α on NOD/SCID engraftment were diminished. This work confirms that AML is influenced by the SDF-1α/CXCR4 axis and demonstrates that disruption of this axis by the bicyclam AMD3100 can influence AML microenvironmental interactions.",

keywords = "AMD3100, Acute leukemia, CXCR4/SDF-1α, Transmigration",

author = "Liesveld, {Jane L.} and Jeremy Bechelli and Karen Rosell and Chaohui Lu and Gary Bridger and Gordon Phillips and Abboud, {Camille N.}",

note = "Funding Information: This work was supported by in part by R21 CA 112835-02 (J.L.L.) and Strong Memorial Hospital. J.L.L. was the Douglas Kroll Research Foundation Translational Researcher of the Leukemia and Lymphoma Society of America (6030). ",

year = "2007",

month = nov,

doi = "10.1016/j.leukres.2007.02.017",

language = "English",

volume = "31",

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journal = "Leukemia Research",

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T1 - Effects of AMD3100 on transmigration and survival of acute myelogenous leukemia cells

AU - Liesveld, Jane L.

AU - Bechelli, Jeremy

AU - Rosell, Karen

AU - Lu, Chaohui

AU - Bridger, Gary

AU - Phillips, Gordon

AU - Abboud, Camille N.

N1 - Funding Information: This work was supported by in part by R21 CA 112835-02 (J.L.L.) and Strong Memorial Hospital. J.L.L. was the Douglas Kroll Research Foundation Translational Researcher of the Leukemia and Lymphoma Society of America (6030).

PY - 2007/11

Y1 - 2007/11

N2 - Acute myelogenous leukaemia (AML) blasts transmigrate in response to SDF-1α. AMD3100, a novel bicyclam molecule which inhibits stromal-derived factor (SDF)-1α/CXCR4 interactions, inhibited the transmigration of AML blasts and inhibited outgrowth of leukemia colony forming units. AMD3100 did not abrogate stroma-mediated protection from cytarabine-mediated apoptosis, except in the case of one promyelocytic leukemic sample tested, and it did not influence adhesion of blasts to endothelial monolayers. When AML blasts were pretreated with AMD3100, the positive effects of SDF-1α on NOD/SCID engraftment were diminished. This work confirms that AML is influenced by the SDF-1α/CXCR4 axis and demonstrates that disruption of this axis by the bicyclam AMD3100 can influence AML microenvironmental interactions.

AB - Acute myelogenous leukaemia (AML) blasts transmigrate in response to SDF-1α. AMD3100, a novel bicyclam molecule which inhibits stromal-derived factor (SDF)-1α/CXCR4 interactions, inhibited the transmigration of AML blasts and inhibited outgrowth of leukemia colony forming units. AMD3100 did not abrogate stroma-mediated protection from cytarabine-mediated apoptosis, except in the case of one promyelocytic leukemic sample tested, and it did not influence adhesion of blasts to endothelial monolayers. When AML blasts were pretreated with AMD3100, the positive effects of SDF-1α on NOD/SCID engraftment were diminished. This work confirms that AML is influenced by the SDF-1α/CXCR4 axis and demonstrates that disruption of this axis by the bicyclam AMD3100 can influence AML microenvironmental interactions.

KW - AMD3100

KW - Acute leukemia

KW - CXCR4/SDF-1α

KW - Transmigration

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DO - 10.1016/j.leukres.2007.02.017

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C2 - 17403536

AN - SCOPUS:35448936860

SN - 0145-2126

VL - 31

SP - 1553

EP - 1563

JO - Leukemia Research

JF - Leukemia Research

IS - 11

ER -

Effects of AMD3100 on transmigration and survival of acute myelogenous leukemia cells

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