TY - JOUR
T1 - Effect of weight loss on whole body and cellular lipid metabolism in severely obese humans
AU - Klein, S.
AU - Luu, K.
AU - Gasic, S.
AU - Green, A.
PY - 1996
Y1 - 1996
N2 - We evaluated the effect of diet-induced weight loss on whole body and cellular lipid metabolism in persons with severe upper body obesity in two study protocols. In protocol 1, palmitate and glycerol rates of appearance (R(a)) in plasma were determined during basal conditions in seven subjects [initial body mass index (BMI) = 41.3 ± 2.2 kg/m2] before and after 20.4 ± 3.0 kg weight loss. Total glycerol and palmitate R(a) decreased from 231.0 ± 19.4 and 166.2 ± 16.6 μmol/min, respectively, before weight loss to 162.7 ± 9.5 and 105.0 ± 9.7 μmol/min, respectively, after weight loss (P < 0.01). However, glycerol and palmitate R(a) expressed per kilogram fat mass were similar both before and after weight loss. In protocol 2, subcutaneous abdominal adipose tissue was obtained before and after 14.4 ± 2.1 kg weight loss in five subjects (initial BMI = 41.6 ± 2.6 kg/m2). Weight loss caused a 38 ± 8% decrease in adipocyte hormone-sensitive lipase concentration (P < 0.05) but was not associated with any consistent changes in the concentrations of GTP-dependent regulatory proteins, G(i)1α, G(i)2α, and G(s)α. We conclude that diet-induced weight loss ameliorates the increase in basal lipolytic rates in persons with severe upper body obesity. These alterations are associated with changes in cellular hormone-sensitive lipase but not GTP-dependent regulatory protein concentrations.
AB - We evaluated the effect of diet-induced weight loss on whole body and cellular lipid metabolism in persons with severe upper body obesity in two study protocols. In protocol 1, palmitate and glycerol rates of appearance (R(a)) in plasma were determined during basal conditions in seven subjects [initial body mass index (BMI) = 41.3 ± 2.2 kg/m2] before and after 20.4 ± 3.0 kg weight loss. Total glycerol and palmitate R(a) decreased from 231.0 ± 19.4 and 166.2 ± 16.6 μmol/min, respectively, before weight loss to 162.7 ± 9.5 and 105.0 ± 9.7 μmol/min, respectively, after weight loss (P < 0.01). However, glycerol and palmitate R(a) expressed per kilogram fat mass were similar both before and after weight loss. In protocol 2, subcutaneous abdominal adipose tissue was obtained before and after 14.4 ± 2.1 kg weight loss in five subjects (initial BMI = 41.6 ± 2.6 kg/m2). Weight loss caused a 38 ± 8% decrease in adipocyte hormone-sensitive lipase concentration (P < 0.05) but was not associated with any consistent changes in the concentrations of GTP-dependent regulatory proteins, G(i)1α, G(i)2α, and G(s)α. We conclude that diet-induced weight loss ameliorates the increase in basal lipolytic rates in persons with severe upper body obesity. These alterations are associated with changes in cellular hormone-sensitive lipase but not GTP-dependent regulatory protein concentrations.
KW - G proteins
KW - fatty acids
KW - glycerol
KW - hormone-sensitive lipase
KW - stable isotopes
UR - http://www.scopus.com/inward/record.url?scp=0029898674&partnerID=8YFLogxK
U2 - 10.1152/ajpendo.1996.270.5.e739
DO - 10.1152/ajpendo.1996.270.5.e739
M3 - Article
C2 - 8967459
AN - SCOPUS:0029898674
SN - 0193-1849
VL - 270
SP - E739-E745
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 5 33-5
ER -